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Reduced Sensitivity of Commercial Spike-Specific Antibody Assays after Primary Infection with the SARS-CoV-2 Omicron Variant.
Springer, David Niklas; Perkmann, Thomas; Jani, Claudia Maria; Mucher, Patrick; Prüger, Katja; Marculescu, Rodrig; Reuberger, Elisabeth; Camp, Jeremy Vann; Graninger, Marianne; Borsodi, Christian; Deutsch, Josef; Lammel, Oliver; Aberle, Stephan Walter; Puchhammer-Stöckl, Elisabeth; Haslacher, Helmuth; Höltl, Eva; Aberle, Judith Helene; Stiasny, Karin; Weseslindtner, Lukas.
  • Springer DN; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Perkmann T; Department of Laboratory Medicine, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Jani CM; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Mucher P; Department of Laboratory Medicine, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Prüger K; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Marculescu R; Department of Laboratory Medicine, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Reuberger E; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Camp JV; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Graninger M; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Borsodi C; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Deutsch J; Private Practice, Völkermarkt, Austria.
  • Lammel O; Private Practice, Ramsau am Dachstein, Austria.
  • Aberle SW; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Puchhammer-Stöckl E; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Haslacher H; Department of Laboratory Medicine, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Höltl E; Center for Public Health, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Aberle JH; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Stiasny K; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
  • Weseslindtner L; Center for Virology, Medical University of Viennagrid.22937.3d, Vienna, Austria.
Microbiol Spectr ; 10(5): e0212922, 2022 Oct 26.
Article in English | MEDLINE | ID: covidwho-2019796
ABSTRACT
The SARS-CoV-2 Omicron variant is characterized by substantial changes in the antigenic structure of the Spike (S) protein. Therefore, antibodies induced by primary Omicron infection lack neutralizing activity against earlier variants. In this study, we analyzed whether these antigenic changes impact the sensitivity of commercial anti-SARS-CoV-2 antibody assays. Sera from 37 unvaccinated, convalescent individuals after putative primary Omicron infection were tested with a panel of 20 commercial anti-SARS-CoV-2 immunoassays. As controls, we used samples from 43 individuals after primary infection with the SARS-CoV-2 ancestral wild-type strain. In addition, variant-specific live-virus neutralization assays were used as a reference for the presence of SARS-CoV-2-specific antibodies in the samples. Notably, in Omicron convalescents, there was a statistically significant reduction in the sensitivity of all antibody assays containing S or its receptor-binding-domain (RBD) as antigens. Furthermore, antibody levels quantified by these assays displayed a weaker correlation with Omicron-specific neutralizing antibody titers than with those against the wild type. In contrast, the sensitivity of nucleocapsid-protein-specific immunoassays was similar in wild-type and Omicron-infected subjects. In summary, the antigenic changes in the Omicron S lead to reduced immunoreactivity in the current commercial S- and RBD-specific antibody assays, impairing their diagnostic performance. IMPORTANCE This study demonstrates that the antigenic changes of the SARS-CoV-2 Omicron variant affect test results from commercial Spike- and RBD-specific antibody assays, significantly diminishing their sensitivities and diagnostic abilities to assess neutralizing antibodies.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Diagnostic study Topics: Variants Limits: Humans Language: English Journal: Microbiol Spectr Year: 2022 Document Type: Article Affiliation country: Spectrum.02129-22

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Diagnostic study Topics: Variants Limits: Humans Language: English Journal: Microbiol Spectr Year: 2022 Document Type: Article Affiliation country: Spectrum.02129-22