Molecular survey of infectious bronchitis virus on poultry farms in Gifu Prefecture, Japan from 2021 to 2022 by RT-PCR with an enhanced level of detection sensitivity for the S1 gene.
J Vet Med Sci
; 84(9): 1157-1163, 2022 Sep 01.
Article
in English
| MEDLINE | ID: covidwho-2021433
ABSTRACT
Infectious bronchitis virus (IBV) is the causative agent of infectious bronchitis (IB) in chickens. There is a correlation between cross-protection and percentage of similarity between nucleotide sequences encoding the S1 subunit, which is responsible for generating neutralizing and serotype-specific antibodies. Therefore, RT-PCR is commonly used to amplify the IBV-S1 gene following DNA sequencing in order to predict the efficacy of vaccines against IBV strains. We successfully enhanced the sensitivity for detection of the IBV-S1 gene by second PCR after purification of the 1st RT-PCR product. Using that method, we obtained detailed information on the prevalence of IBV on poultry farms in Gifu Prefecture, Japan. The IBV-S1 gene detection method used in the current study will enable accurate information on the prevalence of IBV in Japan to be obtained.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Poultry Diseases
/
Coronavirus Infections
/
Infectious bronchitis virus
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
/
Randomized controlled trials
Topics:
Vaccines
Limits:
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
J Vet Med Sci
Journal subject:
Veterinary Medicine
Year:
2022
Document Type:
Article
Affiliation country:
Jvms.22-0206
Similar
MEDLINE
...
LILACS
LIS