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Methods to evaluate the impact of SARS-CoV-2 nucleocapsid mutations on antigen detection by rapid diagnostic tests.
Tieman, Bryan C; Kovacs, Stephen; Rodgers, Mary A; Mohaimani, Aurash; Gregory, Svetoslava; Christensen, David; Moore, Jeffrey A; Schurig-Briccio, Lici; Hartnett, James; Kar, Alak; Leeman, Aaron; Palmer, Angel; Rogers, Lauren; Dragoo, Brian; Muszynski, Steven; Noblesmith, Deborah; Gardner, Samantha; Snipe-Bushey, Abigail; Kill, Anna; Luo, Xinxin; Cherukuri, Sneha; Rae, Tracey; Marohnic, Christopher C; Cloherty, Gavin A; Muerhoff, Anthony Scott; Hemken, Philip M.
  • Tieman BC; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Kovacs S; ARDx, Abbott Laboratories, Westbrook, ME 04092, USA.
  • Rodgers MA; ID Core Research, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Mohaimani A; ID Core Research, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Gregory S; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Christensen D; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Moore JA; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Schurig-Briccio L; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Hartnett J; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Kar A; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Leeman A; ARDx, Abbott Laboratories, Westbrook, ME 04092, USA.
  • Palmer A; ARDx, Abbott Laboratories, Westbrook, ME 04092, USA.
  • Rogers L; ARDx, Abbott Laboratories, Westbrook, ME 04092, USA.
  • Dragoo B; ARDx, Abbott Laboratories, Westbrook, ME 04092, USA.
  • Muszynski S; ARDx, Abbott Laboratories, Westbrook, ME 04092, USA.
  • Noblesmith D; ARDx, Abbott Laboratories, Westbrook, ME 04092, USA.
  • Gardner S; ARDx, Abbott Laboratories, Westbrook, ME 04092, USA.
  • Snipe-Bushey A; ARDx, Abbott Laboratories, Westbrook, ME 04092, USA.
  • Kill A; ARDx, Abbott Laboratories, Westbrook, ME 04092, USA.
  • Luo X; ID Core Research, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Cherukuri S; ID Core Research, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Rae T; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Marohnic CC; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Cloherty GA; ID Core Research, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Muerhoff AS; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
  • Hemken PM; Biologics Discovery, Abbott Laboratories, Abbott Park, IL 60064, USA.
Biotechniques ; 73(3): 136-141, 2022 09.
Article in English | MEDLINE | ID: covidwho-2022198
ABSTRACT
Mutations in the nucleocapsid of SARS-CoV-2 may interfere with antigen detection by diagnostic tests. We used several methods to evaluate the effect of various SARS-CoV-2 nucleocapsid mutations on the performance of the Panbio™ and BinaxNOW™ lateral flow rapid antigen tests and a prototype high-throughput immunoassay that utilizes Panbio antibodies. Variant detection was also evaluated by immunoblot and BIAcore™ assay. A panel of 23 recombinant nucleocapsid antigens (rAgs) were produced that included mutations found in circulating SARS-CoV-2 variants, including variants of concern. All mutant rAgs were detected by all assays, at a sensitivity equivalent to wild-type control (Wuhan strain). Thus, using a rAg approach, we found that the SARS-CoV-2 nucleocapsid mutations examined do not directly impact antigen detection or antigen assay performance.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Variants Limits: Humans Language: English Journal: Biotechniques Year: 2022 Document Type: Article Affiliation country: Btn-2022-0020

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Variants Limits: Humans Language: English Journal: Biotechniques Year: 2022 Document Type: Article Affiliation country: Btn-2022-0020