Optimization of process parameter for theaflavin extraction from black tea (Camellia sinensis) as an inhibitor potential of rdrp SARS-CoV-2 using response surface methodology

ABSTRACT

Theaflavins and their derivatives such as theaflavin gallate have shown a broad spectrum of antiviral activity against several viruses, including influenza A B and hepatitis C viruses. Theaflavins could inhibit RdRp activity through blocking the active site in the catalytic pocket of RdRp in SARS-CoV-2, SARS-CoV and MERS-CoV. The extraction process is carried out using distilled water as solvent. The effect of three independent variabels temperature (70-90°C), time (5-25 minutes), and solvent-solid ratio (301 - 501) on the extraction process of theaflavin from black tea were investigated. Response Surface Methodology was applied for optimization of the three process parameters and expressed at the second-order polynomial. The research showed that temperature was the most influencing variable for the theaflavin extraction from black tea (Camellia sinesis). The optimal condition for the extraction of theaflavin from black tea were performed at temperature of 90°C, 25 minutes and solvent solid ratio 501 (ml/g). © 2023 Author(s).