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[Genetic diversity of Siberian bovine coronavirus isolates (Coronaviridae: Coronavirinae: Betacoronavirus-1: Bovine-Like coronaviruses)].
Glotov, A G; Nefedchenko, A V; Yuzhakov, A G; Koteneva, S V; Glotova, T I; Komina, A K; Krasnikov, N Y.
  • Glotov AG; Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science.
  • Nefedchenko AV; Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science.
  • Yuzhakov AG; Federal Scientific Center All-Russian Research Institute of Experimental Veterinary named after K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences.
  • Koteneva SV; Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science.
  • Glotova TI; Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science.
  • Komina AK; Federal Scientific Center All-Russian Research Institute of Experimental Veterinary named after K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences.
  • Krasnikov NY; Federal Scientific Center All-Russian Research Institute of Experimental Veterinary named after K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences.
Vopr Virusol ; 67(6): 465-474, 2023 02 07.
Article in Russian | MEDLINE | ID: covidwho-20236063
ABSTRACT

INTRODUCTION:

Bovine coronaviruses (BCoVs) are causative agents of diarrhea, respiratory diseases in calves and winter cow dysentery. The study of genetic diversity of these viruses is topical issue. The purpose of the research is studying the genetic diversity of BCoV isolates circulating among dairy cattle in Siberia. MATERIALS AND

METHODS:

Specimens used in this study were collected from animals that died or was forcedly slaughtered before the start of the study. The target for amplification were nucleotide sequences of S and N gene regions.

RESULTS:

Based on the results of RT-PCR testing, virus genome was present in 16.3% of samples from calves with diarrheal syndrome and in 9.9% with respiratory syndrome. The nucleotide sequences of S gene region were determined for 18 isolates, and N gene sequences - for 12 isolates. Based on S gene, isolates were divided into two clades each containing two subclades. First subclade of first clade (European line) included 11 isolates. Second one included classic strains Quebec and Mebus, strains from Europe, USA and Korea, but none of sequences from this study belonged to this subclade. 6 isolates belonged to first subclade of second clade (American-Asian line). Second subclade (mixed line) included one isolate. N gene sequences formed two clades, one of them included two subclades. First subclade included 3 isolates (American-Asian line), and second subclade (mixed) included one isolate. Second clade (mixed) included 8 sequences. No differences in phylogenetic grouping between intestinal and respiratory isolates, as well as according to their geographic origin were identified.

CONCLUSION:

The studied population of BCoV isolates is heterogeneous. Nucleotide sequence analysis is a useful tool for studying molecular epidemiology of BCoV. It can be beneficial for choice of vaccines to be used in a particular geographic region.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cattle Diseases / Coronavirus, Bovine / Coronavirus Infections / Coronavirus / Betacoronavirus 1 Type of study: Observational study / Randomized controlled trials Topics: Vaccines Limits: Animals Language: Russian Journal: Vopr Virusol Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cattle Diseases / Coronavirus, Bovine / Coronavirus Infections / Coronavirus / Betacoronavirus 1 Type of study: Observational study / Randomized controlled trials Topics: Vaccines Limits: Animals Language: Russian Journal: Vopr Virusol Year: 2023 Document Type: Article