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A novel S2-derived peptide-based ELISA for broad detection of antibody against infectious bronchitis virus.
Xie, Jing; Meng, Xianchen; Zhang, Jianjun; Xie, Quan; Zhang, Wei; Li, Tuofan; Shao, Hongxia; Wan, Zhimin; Qin, Aijian; Ye, Jianqiang.
  • Xie J; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious
  • Meng X; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious
  • Zhang J; Sinopharm Yangzhou VAC Biological Engineering Co., Ltd., Yangzhou, Jiangsu 225127, China.
  • Xie Q; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious
  • Zhang W; Sinopharm Yangzhou VAC Biological Engineering Co., Ltd., Yangzhou, Jiangsu 225127, China.
  • Li T; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious
  • Shao H; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious
  • Wan Z; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious
  • Qin A; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious
  • Ye J; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious
Poult Sci ; 102(6): 102661, 2023 Jun.
Article in English | MEDLINE | ID: covidwho-20244886
ABSTRACT
Avian infectious bronchitis (IB) is a highly contagious disease caused by infectious bronchitis virus (IBV). Vaccination is an effective approach for controlling IBV. Therefore, reliable immune monitoring for IB is critical for poultry. In this study, a novel peptide derived from S2 protein was used to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of broadly cross-reactive antibodies against IBV. The peptide-based ELISA (pELISA) showed good specificity and sensitivity in detecting IBV antibodies against different serotypes. A semilogarithmic regression method for determining IBV antibody titers was also established. Antibody titers detected by pELISA and calculated with this equation were statistically similar to those evaluated by indirect fluorescence assay (IFA). Moreover, the comparison analysis showed a 96.07% compatibility between the pELISA and IDEXX ELISA. All these data demonstrate that the pELISA generated here can be as a rapid and reliable serological surveillance tool for monitoring IBV infection or vaccination.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Poultry Diseases / Coronavirus Infections / Infectious bronchitis virus Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Topics: Vaccines Limits: Animals Language: English Journal: Poult Sci Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Poultry Diseases / Coronavirus Infections / Infectious bronchitis virus Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Topics: Vaccines Limits: Animals Language: English Journal: Poult Sci Year: 2023 Document Type: Article