Analysis of the proliferation characteristics of porcine deltacoronavirus on suspension cultured porcine kidney cell LLC-PK1
Acta Veterinaria et Zootechnica Sinica
; 53(6):2024-2028, 2022.
Article
in Chinese
| CAB Abstracts | ID: covidwho-2025545
ABSTRACT
This study aimed to analyze the proliferation characteristics of porcine deltacoronavirus (PDCoV) in suspension cultured porcine kidney cells LLC-PK1, so as to provide Candidate cell for large-scale production of PDCoV inactivated vaccine. LLC-PK1 cells were suspended by gradually decreasing serum method. PDCoV adaptive monoclonal cell lines were screened by limited dilution method. Indirect immunofluorescence method was used to identify the infectivity of PDCoV. The initial cell density, MOI, time of receiving virus collection and TPCK pancreatin concentration were screened to determine the best suspension culture conditions. The suspension cell strain LLC-PK1Sa which can proliferate PDCoV efficiently was screened out;PDCoV can specifically infect LLC-PK1 cells;PDCoV inoculated LLC-PK1Sa cells with a density of 2 x 106 cells.mL-1 according to the MOI of 10-3, When the final concentration of TPCK pancreatin reached 7.5 g.mL-1, the titer of virus solution harvested 48 h after inoculation was the highest. In this study, the efficient proliferation of PDCoV in LLC-PK1Sa suspension cells was realized for the first time, and the suspension culture conditions were preliminarily optimized, which could provide theoretical reference for large-scale production of PDCoV inactivated vaccine.
Prion, Viral, Bacterial and Fungal Pathogens of Animals [LL821]; Diagnostic, Therapeutic and Pharmacological Biotechnology [WW700]; Biochemistry and Physiology of Microorganisms [ZZ394]; Genetics and Molecular Biology of Microorganisms [ZZ395]; Animal Immunology [LL650]; Host Resistance and Immunity [HH600]; cell culture; cell lines; characterization; immunofluorescence; inactivated vaccines; infectivity; monoclonal antibodies; pancreatin; vaccines; immunization; kidneys; vaccination; disease prevention; disease control; immune sensitization; Porcine deltacoronavirus; pigs; Deltacoronavirus; Orthocoronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; Sus scrofa; Sus; Suidae; Suiformes; Artiodactyla; mammals; vertebrates; Chordata; animals; eukaryotes; fluorescent antibody technique; IFAT; killed vaccines; swine; hogs
Full text:
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Collection:
Databases of international organizations
Database:
CAB Abstracts
Language:
Chinese
Journal:
Acta Veterinaria et Zootechnica Sinica
Year:
2022
Document Type:
Article
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