Isothermal amplification technology for diagnosis of COVID-19: current status and future prospects
Zoonoses
; 2(5), 2022.
Article
in English
| CAB Abstracts | ID: covidwho-2025745
ABSTRACT
During the COVID-19 pandemic, polymerase chain reaction (PCR) has become the gold standard for the detection of SARS-CoV-2 RNA worldwide. However, PCR-based nucleic acid detection technology remains relatively time-consuming, and requires specialized instrumentation and technical personnel;therefore, PCR is difficult to apply at primary-level medical institutions. Antibody-based detection has limitations because of the late appearance of antibodies, thus making early diagnosis difficult, whereas antigen-based detection has insufficient sensitivity, thus resulting in a high false-negative rate. Here, we briefly summarize the development and applications of the nucleic acid isothermal amplification technique (IAT) and describe four major IATs used for the detection of SARS-CoV-2 RNA in mainland China, which have been officially approved by the National Medical Products Administration. In particular, we elaborate on the strengths and weakness of the different IAT in practical settings. We also discuss the outlook for IAT development and propose considerations for the future use of IATs in China.
Prion, Viral, Bacterial and Fungal Pathogens of Humans [VV210]; Diagnosis of Human Disease [VV720]; Techniques and Methodology [ZZ900]; Molecular Biology and Molecular Genetics [ZZ360]; coronavirus disease 2019; diagnosis; diagnostic techniques; early diagnosis; human diseases; molecular genetics techniques; nucleic acids; polymerase chain reaction; public health; RNA; screening; viral diseases; man; Severe acute respiratory syndrome coronavirus 2; China; APEC countries; East Asia; Asia; high Human Development Index countries; upper-middle income countries; Homo; Hominidae; primates; mammals; vertebrates; Chordata; animals; eukaryotes; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; People's Republic of China; PCR; ribonucleic acid; screening tests; SARS-CoV-2; viral infections
Full text:
Available
Collection:
Databases of international organizations
Database:
CAB Abstracts
Language:
English
Journal:
Zoonoses
Year:
2022
Document Type:
Article
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