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Tanshinone IIA loaded bioactive nanoemulsion for alleviation of lipopolysaccharide induced acute lung injury via inhibition of endothelial glycocalyx shedding.
El-Moslemany, Riham M; El-Kamel, Amal H; Allam, Eman A; Khalifa, Hoda M; Hussein, Ahmed; Ashour, Asmaa A.
  • El-Moslemany RM; Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt. Electronic address: riham.elmoslemany@alexu.edu.eg.
  • El-Kamel AH; Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt.
  • Allam EA; Department of Medical Physiology, Faculty of Medicine, Alexandria University, Alexandria 21131, Egypt.
  • Khalifa HM; Department of Histology, Faculty of Medicine, Alexandria University, Alexandria 21131, Egypt.
  • Hussein A; Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Egypt.
  • Ashour AA; Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt.
Biomed Pharmacother ; 155: 113666, 2022 Nov.
Article in English | MEDLINE | ID: covidwho-2027928
ABSTRACT
Acute lung injury (ALI) and its more serious form; acute respiratory distress syndrome are major causes of COVID-19 related mortality. Finding new therapeutic targets for ALI is thus of great interest. This work aimed to prepare a biocompatible nanoformulation for effective pulmonary delivery of the herbal drug; tanshinone-IIA (TSIIA) for ALI management. A nanoemulsion (NE) formulation based on bioactive natural ingredients; rhamnolipid biosurfactant and tea-tree oil, was developed using a simple ultrasonication technique, optimized by varying oil concentration and surfactantoil ratio. The selected TSIIA-NE formulation showed 105.7 nm diameter and a PDI âˆ¼ 0.3. EE exceeded 98 % with biphasic sustained drug release and good stability over 3-months. In-vivo efficacy was evaluated in lipopolysaccharide (LPS)-induced ALI model. TSIIA-NE (30 µg/kg) was administered once intratracheally 2 h after LPS instillation. Evaluation was performed 7days post-treatment. Pulmonary function assessment, inflammatory, oxidative stress and glycocalyx shedding markers analysis in addition to histopathological examination of lung tissue were performed. When compared to untreated rats, in-vivo efficacy study demonstrated 1.4 and 1.9-fold increases in tidal volume and minute respiratory volume, respectively, with 32 % drop in wet/dry lung weight ratio and improved levels of arterial blood gases. Lung histopathology and biochemical analysis of different biomarkers in tissue homogenate and bronchoalveolar lavage fluid indicated that treatment may ameliorate LPS-induced ALI symptoms thorough anti-oxidative, anti-inflammatory effects and inhibition of glycocalyx degradation. TSIIA-NE efficacy was superior to free medication and blank-NE. The enhanced efficacy of TSIIA bioactive nanoemulsion significantly suggests the pharmacotherapeutic potential of bioactive TSIIA-NE as a promising nanoplatform for ALI.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Acute Lung Injury / COVID-19 Drug Treatment Type of study: Experimental Studies Topics: Traditional medicine Limits: Animals Language: English Journal: Biomed Pharmacother Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Acute Lung Injury / COVID-19 Drug Treatment Type of study: Experimental Studies Topics: Traditional medicine Limits: Animals Language: English Journal: Biomed Pharmacother Year: 2022 Document Type: Article