Actin cytoskeleton remodeling primes RIG-I-like receptor activation.
Cell
; 185(19): 3588-3602.e21, 2022 Sep 15.
Article
in English
| MEDLINE | ID: covidwho-2027949
ABSTRACT
The current dogma of RNA-mediated innate immunity is that sensing of immunostimulatory RNA ligands is sufficient for the activation of intracellular sensors and induction of interferon (IFN) responses. Here, we report that actin cytoskeleton disturbance primes RIG-I-like receptor (RLR) activation. Actin cytoskeleton rearrangement induced by virus infection or commonly used reagents to intracellularly deliver RNA triggers the relocalization of PPP1R12C, a regulatory subunit of the protein phosphatase-1 (PP1), from filamentous actin to cytoplasmic RLRs. This allows dephosphorylation-mediated RLR priming and, together with the RNA agonist, induces effective RLR downstream signaling. Genetic ablation of PPP1R12C impairs antiviral responses and enhances susceptibility to infection with several RNA viruses including SARS-CoV-2, influenza virus, picornavirus, and vesicular stomatitis virus. Our work identifies actin cytoskeleton disturbance as a priming signal for RLR-mediated innate immunity, which may open avenues for antiviral or adjuvant design.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Actins
/
COVID-19
Limits:
Humans
Language:
English
Journal:
Cell
Year:
2022
Document Type:
Article
Affiliation country:
J.cell.2022.08.011
Similar
MEDLINE
...
LILACS
LIS