False positive serology of prepandemic chagasic samples with SARS-CoV-2 antigen.
Trop Med Int Health
; 27(11): 1009-1012, 2022 11.
Article
in English
| MEDLINE | ID: covidwho-2029427
ABSTRACT
OBJECTIVE:
To determine whether prepandemic sera from patients with Chagas disease recognise SARS-CoV-2 antigens. MATERIALS ANDMETHODS:
Forty sera from patients with Chagas disease were tested for the presence of IgG cross-reactivity against the nucleocapsid protein (NP) and spike (S) SARS-CoV-2 proteins by ELISA. Positive samples were tested again using a different ELISA and CLIA, both against NP.RESULTS:
None of the sera from patients with Chagas disease, previously confirmed as positive for the presence of anti-Trypanosoma cruzi antibodies reacted against the SARS-CoV-2 S protein, and six samples tested positive for the NP antigen (15%). The six positive samples were re-tested, five remained positive by ELISA and all were negative by CLIA.CONCLUSION:
According to our data, false-positive results might be a concern in the detection of SARS-CoV-2 antibodies in patients with Chagas disease.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Chagas Disease
/
COVID-19
Type of study:
Diagnostic study
/
Randomized controlled trials
Limits:
Humans
Language:
English
Journal:
Trop Med Int Health
Journal subject:
Tropical Medicine
/
Public Health
Year:
2022
Document Type:
Article
Affiliation country:
Tmi.13818
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