Validation Study of a Direct Real-Time PCR Protocol for Detection of Monkeypox Virus.
J Mol Diagn
; 24(11): 1155-1159, 2022 Nov.
Article
in English
| MEDLINE | ID: covidwho-2031470
ABSTRACT
Monkeypox has recently been described as a public health emergency of international concern by the World Health Organization and a public health emergency by the United States. If the outbreak continues to grow, rapid scalability of laboratory testing will be imperative. During the early days of the coronavirus disease 2019 (COVID-19) pandemic, laboratories improved the scalability of testing by using a direct-to-PCR approach. To improve the scalability of monkeypox testing, a direct real-time PCR protocol for the detection of monkeypox virus was validated. The assay retains the sensitivity and accuracy of the indirect assay while eliminating the need for nucleic acid extraction kits, reducing laboratory technologist time per sample and decreasing exposure to an infectious agent. The direct method will make it easier for laboratories across the world to rapidly develop, validate, and scale testing for monkeypox virus.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Monkeypox
/
COVID-19
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Limits:
Humans
Country/Region as subject:
North America
Language:
English
Journal:
J Mol Diagn
Journal subject:
Molecular Biology
Year:
2022
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS