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DEAD-box RNA helicase 21 negatively regulates cytosolic RNA-mediated innate immune signaling.
Li, Jia; Fang, Puxian; Zhou, Yanrong; Wang, Dang; Fang, Liurong; Xiao, Shaobo.
  • Li J; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
  • Fang P; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
  • Zhou Y; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
  • Wang D; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
  • Fang L; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
  • Xiao S; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
Front Immunol ; 13: 956794, 2022.
Article in English | MEDLINE | ID: covidwho-2032775
ABSTRACT
DEAD-box RNA helicase 21 (DDX21), also known as RHII/Gu, is an ATP-dependent RNA helicase. In addition to playing a vital role in regulating cellular RNA splicing, transcription, and translation, accumulated evidence has suggested that DDX21 is also involved in the regulation of innate immunity. However, whether DDX21 induces or antagonizes type I interferon (IFN-I) production has not been clear and most studies have been performed through ectopic overexpression or RNA interference-mediated knockdown. In this study, we generated DDX21 knockout cell lines and found that knockout of DDX21 enhanced Sendai virus (SeV)-induced IFN-ß production and IFN-stimulated gene (ISG) expression, suggesting that DDX21 is a negative regulator of IFN-ß. Mechanistically, DDX21 competes with retinoic acid-inducible gene I (RIG-I) for binding to double-stranded RNA (dsRNA), thereby attenuating RIG-I-mediated IFN-ß production. We also identified that the 217-784 amino acid region of DDX21 is essential for binding dsRNA and associated with its ability to antagonize IFN production. Taken together, our results clearly demonstrated that DDX21 negatively regulates IFN-ß production and functions to maintain immune homeostasis.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Double-Stranded / Interferon-beta Language: English Journal: Front Immunol Year: 2022 Document Type: Article Affiliation country: Fimmu.2022.956794

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Double-Stranded / Interferon-beta Language: English Journal: Front Immunol Year: 2022 Document Type: Article Affiliation country: Fimmu.2022.956794