Immunostimulating Study of Active Agent Fraction from Sungkai (Peronema canescens Jack.) Leaf from SARS-COV-2 Virus Antigen Exposure to NK and CD8+T Cells
Pharmacognosy Journal
; 14(4):344-351, 2022.
Article
in English
| EMBASE | ID: covidwho-2033370
ABSTRACT
Introduction:
Sungkai (Peronema canescens Jack.) plant had been used as an immune system enhancer.Aim:
In this study, the effect of Sungkai leaf extracts from 4 different fractions, namely n-hexane, ethyl acetate, butanol and residual water with 3 variations in doses of 1,10 and 100 mg/kg bw on the activity of NK and CD8+T cells in male white mice that have been exposed to SARS-Cov-2 virus antigen was investigated.Methods:
The experimental animals used were 60 animals divided into 12 groups with 14 days of treatment which had previously been induced with SARS-Cov-2 virus antigen (Moderna) and given with Sungkai leaf extracts for 14 days and evaluated on day 15. The evaluation results of NK cells concentrations sequentially were 2.96;4.66;5.38;5.43;4.05;2.89;3.56;4.21;2.88;1.99;2.07;4.40;3.21;3.40;and 6.93 ng/ml. On the other hand, the evaluation results of CD8+T cells concentrations sequentially were 27.47;28.96;29.19;27.90;21.85;25.79;27.98;23.50;23.39;26.56;22.62;25.19;23,55;26,75;and 29,69 ng/ml. One-way ANOVA and Duncan test were used for the data analysis.Results:
The results showed significant increase of concentration (p<0.05) towards concentration of NK cells in the butanol fraction at a dose of 1 mg/kg BW and CD8+T cells in the residual water fraction at a dose of 100 mg/kg BW.Conclusion:
It can be concluded that fraction from sungkai (Peronema canescens Jack.) at doses of 1,10 and 100 mg/kg bw shows immunostimulatory activity.
adult; analysis of variance; animal cell; animal experiment; article; CD8+ T lymphocyte; controlled study; data analysis; human; male; mouse; natural killer cell; natural killer cell mediated cytotoxicity; nonhuman; plant leaf; Severe acute respiratory syndrome coronavirus 2; acetic acid ethyl ester; butanol; hexane; plant extract; virus antigen; water
Full text:
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Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
Pharmacognosy Journal
Year:
2022
Document Type:
Article
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