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Feasibility and accuracy verification of respiratory virus detection by mask virus extraction
Chinese Journal of Nosocomiology ; 32(12):1880-1884, 2022.
Article in English, Chinese | CAB Abstracts | ID: covidwho-2034518
ABSTRACT

OBJECTIVE:

To explore a new method for detecting respiratory viruses by extracting residual virus on mask, and verify its reliability and sensitivity.

METHODS:

The novel coronavirus analogs-s La Sota strains of chicken Newcastle disease virus and H120 strains of infectious bronchitis virus with different diluted concentrations were sprayed onto surgical masks and N95 masks through a respiratory simulator, and they were left standing at room temperature for 2 hours and 12 hours, respectively. The cDNA and its amplification cycle(CT) values of the nucleoocapsid protein(N) of chicken Newcastle disease virus and the nucleoprotein(NP) genes of infectious bronchitis virus were detected by ordinary polymerase chain reaction(PCR) and quantitative real-time PCR(qRT-PCR). The minimum detectable virus concentration and viral content in masks under different retention times were compared.

RESULTS:

The gene bands of the Newcastle disease virus La Sota strains and the infectious bronchitis virus H120 strains were detected on the masks stored for different times, and the total RNA of the virus had good amplification curves in the range of 10 pg-10 ng. The mean CT values of N gene and NP gene of the residual virus on the general medical surgical mask and N95 masks placed for 2 h were 22.547+or-0.342,23.698+or-0.501 and 22.855+or-0.308,24.036+or-0.338, respectively. However, only part of them could be detected after 12 h. respectively, and there was no significant difference in CT values between the two masks during the same period of time(P2 h=0.452, P12 h=0.355). The minimum detectable concentration of virus in the masks was 1800, and the number of residual viruses on the mask that can be detected was 6.75x10~3.

CONCLUSION:

The method of screening coronavirus by detecting virus residues on masks within 2 hours was feasible and suitable for medical surgical masks and N95 masks, which can be used for preliminary screening of respiratory viruses.
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Collection: Databases of international organizations Database: CAB Abstracts Language: English / Chinese Journal: Chinese Journal of Nosocomiology Year: 2022 Document Type: Article

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Collection: Databases of international organizations Database: CAB Abstracts Language: English / Chinese Journal: Chinese Journal of Nosocomiology Year: 2022 Document Type: Article