Genotyping, virulence genes and drug resistance of MRSA strains isolated from skin and soft tissue infection in Tianjin
Chinese Journal of Nosocomiology
; 32(12):1812-1816, 2022.
Article
in English, Chinese
| GIM | ID: covidwho-2034536
ABSTRACT
OBJECTIVE:
To investigate and analyze the genotyping, virulence genes and drug-resistant genes of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from skin and soft tissue infections in this area.METHODS:
The skin secretions of 204 patients with skin and soft tissue infections in the Fifth Central Hospital of Tianjin between Jan. 2019 and Dec. 2020 were collected, and MRSA strains identified as non-repetitive strains were isolated. The Staphylococcal cassette chromosome mec (SCCmec) and Staphylococcal protein A gene (spa) genotyping and Panton-valentine leukocidin (PVL) gene carrying status were analyzed among the MRSA strains, and their relationship with drug resistance was analyzed.RESULTS:
Totally 82 strains of S. aureus were isolated from the skin secretions of 204 patients with skin and soft tissue infections, including 44 strains of MRSA (53.66%). The most common SCCmec genotype was genotype III (accounting for 84.09%) and the most common spa genotype was genotype t030 (accounting for 84.09%). PVL genes encoding virulence factors were amplified in 5 strains (11.36%). The drug resistance rates of 44 MRSA strains to vancomycin and compound sulfamethoxazole were 0.00%, and all the strains were drug-resistant to penicillin. Different SCCmec and spa genotypes were highly resistant to erythromycin, cefazolin, clindamycin and levofloxacin, but the differences in drug resistance rates of different SCCmec genotypes to clindamycin and levofloxacin were significant (P < 0.05). The resistance rates of strains with PVL positive genes to chloramphenicol, gentamicin and tetracycline were significantly higher than those with PVL negative genes (P < 0.05).CONCLUSION:
Strains carrying SCCmec III and spa t030 genotypes may be the dominant strains of MRSA in skin and soft tissue infections in this area. Spa genotypes and PVL gene have certain impact on drug resistance of MRSA, and the isolated MRSA strains are all sensitive to vancomycin and compound sulfamethoxazole, which can provide a reference for anti-MRSA treatment in this area.
18323-44-9; 100986-85-4; 1403-66-3; 1405-41-0; 56-75-7; drug resistance; strains; genes; clindamycin; multiple drug resistance; human diseases; genotypes; viral diseases; coronavirus disease 2019; protein; skin; skin diseases; soft tissue infections; soft tissues; infectious diseases; bacterial diseases; levofloxacin; gentamicin; chloramphenicol; tetracyclines; drug therapy; lincosamide antibiotics; antibiotics; fluoroquinolone antibiotics; aminoglycoside antibiotics; methicillin-resistant Staphylococcus aureus; man; Severe acute respiratory syndrome coronavirus 2; Staphylococcus; Staphylococcus aureus; Tianjin; China; Staphylococcaceae; Bacillales; Bacilli; Firmicutes; Bacteria; prokaryotes; Homo; Hominidae; primates; mammals; vertebrates; Chordata; animals; eukaryotes; Northern China; APEC countries; East Asia; Asia; high Human Development Index countries; upper-middle income countries; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; Mrsa; Tientsin; SARS-CoV-2; viral infections; dermis; dermatoses; communicable diseases; bacterial infections; bacterioses; bacterium; chemotherapy; People's Republic of China
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Collection:
Databases of international organizations
Database:
GIM
Language:
English
/
Chinese
Journal:
Chinese Journal of Nosocomiology
Year:
2022
Document Type:
Article
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