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Selective reversed-phase high-performance liquid chromatography method for the determination of intact SARS-CoV-2 spike protein.
Lorbetskie, Barry; White, Taryn; Creskey, Marybeth; Zhang, Xu; Girard, Michel; Tam, Roger Y; Sauvé, Simon; Lu, Huixin.
  • Lorbetskie B; Separation Sciences and Analytical Biotechnology Laboratory, Biologics and Radiopharmaceutical Drugs Directorate, Health Canada, 251 - Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2.
  • White T; Separation Sciences and Analytical Biotechnology Laboratory, Biologics and Radiopharmaceutical Drugs Directorate, Health Canada, 251 - Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2.
  • Creskey M; Separation Sciences and Analytical Biotechnology Laboratory, Biologics and Radiopharmaceutical Drugs Directorate, Health Canada, 251 - Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2.
  • Zhang X; Separation Sciences and Analytical Biotechnology Laboratory, Biologics and Radiopharmaceutical Drugs Directorate, Health Canada, 251 - Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2.
  • Girard M; Separation Sciences and Analytical Biotechnology Laboratory, Biologics and Radiopharmaceutical Drugs Directorate, Health Canada, 251 - Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2; Scientist Emeritus, Health Canada.
  • Tam RY; Separation Sciences and Analytical Biotechnology Laboratory, Biologics and Radiopharmaceutical Drugs Directorate, Health Canada, 251 - Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2.
  • Sauvé S; Separation Sciences and Analytical Biotechnology Laboratory, Biologics and Radiopharmaceutical Drugs Directorate, Health Canada, 251 - Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2.
  • Lu H; Separation Sciences and Analytical Biotechnology Laboratory, Biologics and Radiopharmaceutical Drugs Directorate, Health Canada, 251 - Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2. Electronic address: huixin.lu@hc-sc.gc.ca.
J Chromatogr A ; 1680: 463424, 2022 Sep 13.
Article in English | MEDLINE | ID: covidwho-2035853
ABSTRACT
Protein-based vaccines are playing an increasingly important role in the COVID-19 pandemic. As late-stage clinical data are finalized and released, the number of protein-based vaccines expected to enter the market will increase significantly. Most protein-based COVID-19 vaccines are based on the SARS-CoV-2 spike protein (S-protein), which plays a major role in viral attachment to human cells and infection. As a result, in order to develop and manufacture quality vaccines consistently, it is imperative to have access to selective and efficient methods for the bioanalytical assessment of S-protein. In this study, samples of recombinant S-protein (hexS-protein) and commercial S-protein were used to develop a selective reversed-phase HPLC (RP-HPLC) method that enabled elution of the intact S-protein monomer as a single peak on a wide pore, C8-bonded chromatographic column. The S-protein subunits, S1 and S2 subunits, were clearly separated from intact S-protein and identified. The results of this study set the foundation for reversed-phase HPLC method development and analysis for selective and efficient separation of S-protein monomer from its subunits.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: J Chromatogr A Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: J Chromatogr A Year: 2022 Document Type: Article