Evaluation of sample pooling method for SARS CoV-2 RNA detection;A laboratory based simulated study
Sri Lankan Journal of Infectious Diseases
; 12(2), 2022.
Article
in English
| CAB Abstracts | ID: covidwho-2040070
ABSTRACT
Real time RT-PCR is considered as the gold standard test to detect COVID-19. The use of sample pooling strategy increases testing capacity and spares resources. However, the effectiveness of sample pooling should be evaluated in the setting before being implemented. Forty five samples including 20 high positives (Ct<20), 20 low positives (Ct 20-40) and 05 negative samples were used to prepare 11, 13 and 15 simulated sample pools which were then subjected to viral RNA extraction followed by real time RT-PCR. Sensitivity and specificity of sample pooling technique in the detection of SARS-CoV-2 RNA was 100% without significant variation of Ct values. According to our results, pooling of up to 6 samples will not have an effect on the final result in clinical samples and hence can be adopted in the given context for the diagnosis of COVID-19 by RT-PCR.
Diagnosis of Human Disease [VV720]; Prion, Viral, Bacterial and Fungal Pathogens of Humans [VV210]; Molecular Biology and Molecular Genetics [ZZ360]; Genetics and Molecular Biology of Microorganisms [ZZ395]; Techniques and Methodology [ZZ900]; coronavirus disease 2019; diagnosis; diagnostic techniques; genetic sequencing; human diseases; laboratories; laboratory diagnosis; laboratory methods; nucleotide sequences; real time PCR; reverse transcriptase PCR; RNA; screening; viral diseases; methodology; man; Severe acute respiratory syndrome coronavirus 2; Homo; Hominidae; primates; mammals; vertebrates; Chordata; animals; eukaryotes; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; laboratory techniques; DNA sequences; reverse transcriptase polymerase chain reaction; RT-PCR; ribonucleic acid; screening tests; SARS-CoV-2; viral infections; methods
Full text:
Available
Collection:
Databases of international organizations
Database:
CAB Abstracts
Type of study:
Experimental Studies
Language:
English
Journal:
Sri Lankan Journal of Infectious Diseases
Year:
2022
Document Type:
Article
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