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Establishment and preliminary application of a TaqMan real-time quantitative PCR method for PastV3 detection
Chinese Veterinary Science / Zhongguo Shouyi Kexue ; 50(11):1373-1378, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-2040499
ABSTRACT
In order to build a specific, sensitive and rapid detection method for PAstV3 detection, the PAstVB gene sequences in Genbank were used and the conserved region in ORFlb was selected to design specific primers and TaqMan probe. Clinical stool samples were collected and preliminary detected by this newly established real-time RT-PCR method after reaction systems and conditions optimization. This detection method established in this study has a good linear relationship with the standard curve, with R2 value up to 0.9971. The sensitivity is 100 times higher than conventional PCR method, The variation co-efficient of in-batch and inter-batch repeatability test is less than 2.0%, indicating good repeatability. The detection results of Clinical samples showed that the positive rate of this method is higher than conventional PCR method. The establishment of this method provides a rapid detection means for PAstV3 laboratory diagnosis and epidemiological investigation.
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Full text: Available Collection: Databases of international organizations Database: CAB Abstracts Type of study: Diagnostic study / Prognostic study Language: Chinese Journal: Zhongguo Shouyi Kexue Year: 2020 Document Type: Article

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Full text: Available Collection: Databases of international organizations Database: CAB Abstracts Type of study: Diagnostic study / Prognostic study Language: Chinese Journal: Zhongguo Shouyi Kexue Year: 2020 Document Type: Article