Establishment and preliminary application of a TaqMan real-time quantitative PCR method for PastV3 detection
Chinese Veterinary Science / Zhongguo Shouyi Kexue
; 50(11):1373-1378, 2020.
Article
in Chinese
| CAB Abstracts | ID: covidwho-2040499
ABSTRACT
In order to build a specific, sensitive and rapid detection method for PAstV3 detection, the PAstVB gene sequences in Genbank were used and the conserved region in ORFlb was selected to design specific primers and TaqMan probe. Clinical stool samples were collected and preliminary detected by this newly established real-time RT-PCR method after reaction systems and conditions optimization. This detection method established in this study has a good linear relationship with the standard curve, with R2 value up to 0.9971. The sensitivity is 100 times higher than conventional PCR method, The variation co-efficient of in-batch and inter-batch repeatability test is less than 2.0%, indicating good repeatability. The detection results of Clinical samples showed that the positive rate of this method is higher than conventional PCR method. The establishment of this method provides a rapid detection means for PAstV3 laboratory diagnosis and epidemiological investigation.
Prion, Viral, Bacterial and Fungal Pathogens of Animals [LL821]; Diagnosis of Animal Diseases [LL886]; Animal Immunology [LL650]; Genetics and Molecular Biology of Microorganisms [ZZ395]; Molecular Biology and Molecular Genetics [ZZ360]; Taxonomy and Evolution [ZZ380]; real time PCR; detection; diagnostic techniques; rapid methods; polymerase chain reaction; swine diseases; viral diseases; diagnosis; genes; nucleotide sequences; gene banks; open reading frames; laboratory diagnosis; epidemiological surveys; epidemiology; Senecavirus A; Suid herpesvirus 1; Classical swine fever virus; Porcine reproductive and respiratory syndrome virus; Porcine epidemic diarrhea virus; Transmissible gastroenteritis virus; Japanese encephalitis virus; pigs; Varicellovirus; Arterivirus; Senecavirus; Picornaviridae; Picornavirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; Alphaherpesvirinae; Herpesviridae; Herpesvirales; dsDNA Viruses; DNA Viruses; Pestivirus; Flaviviridae; Arteriviridae; Nidovirales; Alphacoronavirus; Coronavirinae; Coronaviridae; Alphacoronavirus 1; Flavivirus; Sus scrofa; Sus; Suidae; Suiformes; Artiodactyla; mammals; vertebrates; Chordata; animals; eukaryotes; Porcine enteric virus; Porcine rotavirus; PCR; pig diseases; viral infections; Aujeszky virus; Swine fever virus; Hog cholera virus; hog cholera; Porcine epidemic diarrhoea virus; DNA sequences; germplasm banks; ORFs; swine; hogs
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Collection:
Databases of international organizations
Database:
CAB Abstracts
Language:
Chinese
Journal:
Zhongguo Shouyi Kexue
Year:
2020
Document Type:
Article
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