Systematic evaluation of plasma signaling cascades by functional proteomics approaches: SARS-CoV-2 infection as model.
Proteomics Clin Appl
; 16(6): e2100100, 2022 Nov.
Article
in English
| MEDLINE | ID: covidwho-2047908
ABSTRACT
PURPOSE:
Acute phase reactants (APRs) play a critical role in inflammation. The difference in their physiological functions or the different dynamic ranges of these proteins in plasma makes it difficult to detect them simultaneously and to use several of these proteins as a tool in clinical practice. EXPERIMENTALDESIGN:
A novel multiplex assay has been designed and optimized to carry out a high-throughput and simultaneous screening of APRs, allowing the detection of each of them at the same time and in their corresponding dynamic range.RESULTS:
Using Sars-CoV-2 infection as a model, it has been possible to profile different patterns of acute phase proteins that vary significantly between healthy and infected patients. In addition, severity profiles (acute respiratory distress syndrome and sepsis) have been established. CONCLUSIONS AND CLINICAL RELEVANCE Differential profiles in acute phase proteins can serve as a diagnostic and prognostic tool, among patient stratification. The design of this new platform for their simultaneous detection paves the way for them to be more extensive use in clinical practice.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Acute-Phase Proteins
/
Acute-Phase Reaction
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
/
Experimental Studies
/
Prognostic study
/
Systematic review/Meta Analysis
Limits:
Humans
Language:
English
Journal:
Proteomics Clin Appl
Journal subject:
Biochemistry
Year:
2022
Document Type:
Article
Affiliation country:
Prca.202100100
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