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An Effective Platform for SARS-CoV-2 Prevention by Combining Neutralization and RNAi Technology
Chinese Journal of Polymer Science (Springer Science & Business Media B.V.) ; : 1-7, 2022.
Article in English | Academic Search Complete | ID: covidwho-2048318
ABSTRACT
At present, the coronavirus disease 2019 (COVID-19) pandemic is a global health crisis. Scientists all over the globe are urgently looking forward to an effective solution to prevent the spread of the epidemic and avoid more casualties at an early date. In this study, we establish an effective platform for the prevention of SARS-CoV-2 by combining the neutralization strategy and RNAi technology. To protect normal cells from infection, the customized cells are constructed to stably express viral antigenic receptor ACE2 on the cell membrane. These modified cells are used as bait for inducing the viral entry. The transcription and replication activities of viral genome are intercepted subsequently by the intracellular shRNAs, which are complementary to the viral gene fragments. A pseudotyped virus reconstructed from the HIV lentivirus is utilized as a virus model, by which we validate the feasibility and effectiveness of our strategy in vitro. Our work establishes an initial model and lays the foundation for future prevention and treatment of various RNA viruses. [ FROM AUTHOR] Copyright of Chinese Journal of Polymer Science (Springer Science & Business Media B.V.) is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)
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Full text: Available Collection: Databases of international organizations Database: Academic Search Complete Type of study: Experimental Studies Language: English Journal: Chinese Journal of Polymer Science (Springer Science & Business Media B.V.) Year: 2022 Document Type: Article

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Full text: Available Collection: Databases of international organizations Database: Academic Search Complete Type of study: Experimental Studies Language: English Journal: Chinese Journal of Polymer Science (Springer Science & Business Media B.V.) Year: 2022 Document Type: Article