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Effects of growth hormone/estrogen/androgen on COVID-19-type proinflammatory responses in normal human lung epithelial BEAS-2B cells.
Zhu, Zemin; Zhao, Zhijian; Chen, Xun; Chu, Zhou; He, Yi; Tan, Yingzheng; Zhou, Juan; Tang, Caixi.
  • Zhu Z; Department of Hepatobiliary and Pancreatic Surgery, Zhuzhou Central Hospital,, Zhuzhou, China.
  • Zhao Z; Department of Hepatobiliary and Pancreatic Surgery, Zhuzhou Central Hospital,, Zhuzhou, China.
  • Chen X; Department of Hepatobiliary and Pancreatic Surgery, Zhuzhou Central Hospital,, Zhuzhou, China.
  • Chu Z; Department of Hepatobiliary and Pancreatic Surgery, Zhuzhou Central Hospital,, Zhuzhou, China.
  • He Y; Department of Cardiovascular Medicine, Zhuzhou Central Hospital, Zhuzhou, China.
  • Tan Y; Department of Infectious Diseases, Zhuzhou Central Hospital, Zhuzhou, China.
  • Zhou J; Department of Infectious Diseases, Zhuzhou Central Hospital, Zhuzhou, China.
  • Tang C; Department of Hepatobiliary and Pancreatic Surgery, Zhuzhou Central Hospital,, Zhuzhou, China. tcx082664@163.com.
BMC Mol Cell Biol ; 23(1): 42, 2022 Sep 29.
Article in English | MEDLINE | ID: covidwho-2053858
ABSTRACT

BACKGROUND:

COVID-19 is a disease caused by SARS-CoV-2, which can cause mild to serious infections in humans. We aimed to explore the effect of growth hormone (GH)/estrogen/androgen in normal human lung epithelial BEAS-2B cells on COVID-19-type proinflammatory responses.

METHODS:

A BEAS-2B COVID-19-like proinflammatory cell model was constructed. After that, the cells were treated with GH, 17ß-estradiol (E2), and testosterone (Tes) for 24 h. CCK-8 assays were utilized to evaluate cell viability. The mRNA expression of ACE2, AGTR1, TMRRSS2, and ISG15 and the protein expression of ACE2, AGTR1, TMRRSS2, and ISG15 were measured by qRT‒PCR and Western blotting, respectively. ELISAs were performed to determine IL-6, MCP-1, MDA and SOD expression. Flow cytometry was used to measure ROS levels. Finally, MAPK/NF-κB pathway-related factor expression was evaluated.

RESULTS:

The COVID-19-type proinflammatory model was successfully constructed, and 1000 ng/mL RBD treatment for 24 h was selected as the condition for the model group for subsequent experiments. After RBD treatment, cell viability decreased, the mRNA expression of ACE2, AGTR1, TMRRSS2, and ISG15 and the protein expression of ACE2, AGTR1, TMRRSS2, and ISG15 increased, IL-6, MCP-1, MDA and ROS levels increased, and MDA levels decreased. The mRNA levels of MAPK14 and RELA increased, but the protein levels did not change significantly. In addition, phospho-MAPK14 and phospho-RELA protein levels were also increased. Among the tested molecules, E2 had the most pronounced effect, followed by GH, while Tes showed the opposite effect.

CONCLUSION:

GH/E2 alleviated inflammation in a COVID-19-type proinflammatory model, but Tes showed the opposite effect.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Mitogen-Activated Protein Kinase 14 / COVID-19 Drug Treatment Type of study: Experimental Studies Limits: Humans Language: English Journal: BMC Mol Cell Biol Year: 2022 Document Type: Article Affiliation country: S12860-022-00442-5

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Mitogen-Activated Protein Kinase 14 / COVID-19 Drug Treatment Type of study: Experimental Studies Limits: Humans Language: English Journal: BMC Mol Cell Biol Year: 2022 Document Type: Article Affiliation country: S12860-022-00442-5