K-mer applied in Mycobacterium tuberculosis genome cluster analysis.
Braz J Biol
; 84: e258258, 2022.
Article
in English
| MEDLINE | ID: covidwho-2054598
ABSTRACT
According to studies carried out, approximately 10 million people developed tuberculosis in 2018. Of this total, 1.5 million people died from the disease. To study the behavior of the genome sequences of Mycobacterium tuberculosis (MTB), the bacterium responsible for the development of tuberculosis (TB), an analysis was performed using k-mers (DNA word frequency). The k values ranged from 1 to 10, because the analysis was performed on the full length of the sequences, where each sequence is composed of approximately 4 million base pairs, k values above 10, the analysis is interrupted, as consequence of the program's capacity. The aim of this work was to verify the formation of the phylogenetic tree in each k-mer analyzed. The results showed the formation of distinct groups in some k-mers analyzed, taking into account the threshold line. However, in all groups, the multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains remained together and separated from the other strains.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Tuberculosis, Multidrug-Resistant
/
Extensively Drug-Resistant Tuberculosis
/
Mycobacterium tuberculosis
Limits:
Humans
Language:
English
Journal:
Braz J Biol
Journal subject:
Biology
Year:
2022
Document Type:
Article
Affiliation country:
1519-6984.258258
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