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Understanding Host-Virus Interactions: Assessment of Innate Immune Responses in Mastomys natalensis Cells after Arenavirus Infection.
Brinkmann, Nele Marie; Hoffmann, Chris; Wurr, Stephanie; Pallasch, Elisa; Hinzmann, Julia; Ostermann, Eleonore; Brune, Wolfram; Eskes, Maria Elisabeth; Jungblut, Lukas; Günther, Stephan; Unrau, Ludmilla; Oestereich, Lisa.
  • Brinkmann NM; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
  • Hoffmann C; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
  • Wurr S; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
  • Pallasch E; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
  • Hinzmann J; German Center for Infectious Disease, Partner Site Hamburg-Lübeck-Borstel-Riems, 20359 Hamburg, Germany.
  • Ostermann E; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
  • Brune W; German Center for Infectious Disease, Partner Site Hamburg-Lübeck-Borstel-Riems, 20359 Hamburg, Germany.
  • Eskes ME; Leibniz Institute of Virology, 20359 Hamburg, Germany.
  • Jungblut L; Leibniz Institute of Virology, 20359 Hamburg, Germany.
  • Günther S; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
  • Unrau L; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
  • Oestereich L; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
Viruses ; 14(9)2022 09 08.
Article in English | MEDLINE | ID: covidwho-2055391
ABSTRACT
Mastomys natalensis is the natural host of various arenaviruses, including the human-pathogenic Lassa virus. Homologous arenaviruses, defined here as those having M. natalensis as a natural host, can establish long-lasting infection in M. natalensis, while these animals rapidly clear arenaviruses having another rodent species as a natural host (heterologous viruses). Little is known about the mechanisms behind the underlying arenavirus-host barriers. The innate immune system, particularly the type I interferon (IFN) response, might play a role. In this study, we developed and validated RT-PCR assays to analyse the expression of M. natalensis interferon-stimulated genes (ISGs). We then used these assays to study if homologous and heterologous viruses induce different IFN responses in M. natalensis cells. Infection experiments were performed with the homologous Lassa and Morogoro viruses and the related but heterologous Mobala virus. Compared to the direct induction with IFN or Poly(IC), arenaviruses generally induced a weak IFN response. However, the ISG-expression profiles of homologous and heterologous viruses were similar. Our data indicate that, at least in M. natalensis cells, the IFN system is not a major factor in the virus-host barrier for arenaviruses. Our system provides a valuable tool for future in vivo investigation of arenavirus host restrictions at the level of the innate immune response.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Interferon Type I / Arenavirus / Arenaviridae Infections Type of study: Prognostic study Limits: Animals / Humans Country/Region as subject: Africa Language: English Year: 2022 Document Type: Article Affiliation country: V14091986

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Interferon Type I / Arenavirus / Arenaviridae Infections Type of study: Prognostic study Limits: Animals / Humans Country/Region as subject: Africa Language: English Year: 2022 Document Type: Article Affiliation country: V14091986