Brain Organoids as a Model to Study Zika Virus and SARS-CoV-2 Infections
Neuromethods
; 189:173-190, 2023.
Article
in English
| EMBASE | ID: covidwho-2059680
ABSTRACT
In recent years, we are living through different viral pandemics that result in neurological impairments. Given the human-specific nature of brain development, physiology, and pathology, it is imperative to use human models to investigate the neurological impact of viral infections, such as Zika virus and SARS-CoV-2. Brain organoids are powerful in vitro platforms for the analysis of the effects of viral infections on brain development and function, with prospective application to new emerging viral threats. Using brain organoids, it was possible to show that Zika virus infects neural stem cells, disrupting the cell cycle and neurogenesis, leading to microcephaly, a severe reduction of the brain. On the other hand, while it is still under investigation how SARS-CoV-2 might enter and alter the brain, organoid studies are helping to characterize its neurotropism and potential mechanisms of neurovirulence. Here, we describe a method for the infection of human brain organoid cultures with Zika and Sars-CoV-2 viruses that can be used to study neurodevelopmental phenotypes, alteration in neuronal functionality, host-pathogen interactions, as well as for drug testing. Copyright © 2023, Springer Science+Business Media, LLC, part of Springer Nature.
Brain; covid-19; Development; Human; In vitro; Infection; iPSC; Microcephaly; Organoids; SARS-CoV-2; Zika virus; zikv; cell cycle; cerebral organoid; coronavirus disease 2019; drug screening; host pathogen interaction; human cell; induced pluripotent stem cell; nervous system development; neural stem cell; neurotropism; nonhuman; phenotype; Severe acute respiratory syndrome coronavirus 2
Full text:
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Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
Neuromethods
Year:
2023
Document Type:
Article
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