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Deep sequencing analysis of clinical samples from patients with acute infectious conjunctivitis during the COVID-19 delta surge in Madurai, India.
Lalitha, Prajna; Prajna, N Venkatesh; Gunasekaran, Rameshkumar; Teja, Gonugunta Vishnu; Sharma, Sankalp S; Hinterwirth, Armin; Ruder, Kevin; Zhong, Lina; Chen, Cindi; Deiner, Michael; Huang, ChunHong; Pinsky, Benjamin A; Lietman, Thomas M; Doan, Thuy; Seitzman, Gerami D.
  • Lalitha P; Aravind Eye Hospital, Madurai, India.
  • Prajna NV; Aravind Eye Hospital, Madurai, India.
  • Gunasekaran R; Aravind Eye Hospital, Madurai, India.
  • Teja GV; Aravind Eye Hospital, Madurai, India.
  • Sharma SS; Aravind Eye Hospital, Madurai, India.
  • Hinterwirth A; Francis I. Proctor Foundation, University of California San Francisco, San Francisco, CA, United States.
  • Ruder K; Francis I. Proctor Foundation, University of California San Francisco, San Francisco, CA, United States.
  • Zhong L; Francis I. Proctor Foundation, University of California San Francisco, San Francisco, CA, United States.
  • Chen C; Francis I. Proctor Foundation, University of California San Francisco, San Francisco, CA, United States.
  • Deiner M; Department of Ophthalmology, University of California, San Francisco, CA, United States.
  • Huang C; Department of Pathology, Stanford University School of Medicine, Stanford, CA, United States.
  • Pinsky BA; Department of Pathology, Stanford University School of Medicine, Stanford, CA, United States; Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, CA, United States.
  • Lietman TM; Francis I. Proctor Foundation, University of California San Francisco, San Francisco, CA, United States; Department of Ophthalmology, University of California, San Francisco, CA, United States.
  • Doan T; Francis I. Proctor Foundation, University of California San Francisco, San Francisco, CA, United States; Department of Ophthalmology, University of California, San Francisco, CA, United States. Electronic address: thuy.doan@ucsf.edu.
  • Seitzman GD; Francis I. Proctor Foundation, University of California San Francisco, San Francisco, CA, United States; Department of Ophthalmology, University of California, San Francisco, CA, United States.
J Clin Virol ; 157: 105318, 2022 Dec.
Article in English | MEDLINE | ID: covidwho-2061479
ABSTRACT

BACKGROUND:

Seasonal outbreaks of infectious conjunctivitis remain a public health issue. Determination of outbreak etiologies in the context of a worldwide pandemic may provide useful information to guide public health strategies. The aim of this study was to identify pathogens associated with outpatient infectious conjunctivitis during the COVID-19 Delta surge.

METHODS:

This prospective study was conducted from April 2021 to September 2021. All outpatients presenting to the Aravind Eye Center (Madurai, India) with signs and symptoms consistent with acute infectious conjunctivitis were eligible. Three swabs were obtained from each participant one from each conjunctiva and one from the anterior nares. Samples were processed for metagenomic RNA deep sequencing (RNA-seq).

RESULTS:

Samples from 106 study participants were sequenced. The most common presenting symptoms were tearing (86%) and itching (71%). Preauricular lymphadenopathy was present in 38% of participants. 20% of participants had close contacts with similar symptoms. Systemic symptoms such as coughing, runny nose, vomiting or diarrhea were uncommonly reported. 60% of all participants used some medicated eye drops upon enrollment. 75% of study participants demonstrated infection with human adenovirus D (HAdV-D). 11% of conjunctivitis was associated with SARS-CoV-2. 15% had no definitive pathogen detected. 8% of all participants had codetection of more than one pathogen on RNA-seq.

CONCLUSIONS:

During the COVID-19 Delta surge in India, HAdV-D was the most common pathogen associated with infectious conjunctivitis. SARS-CoV-2 was the second most common associated pathogen. Seasonal surveillance may be necessary for the determination of emerging and reemerging pathogens responsible for infectious conjunctivitis.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Adenoviruses, Human / Conjunctivitis / COVID-19 Type of study: Cohort study / Etiology study / Observational study / Prognostic study Limits: Humans Country/Region as subject: Asia Language: English Journal: J Clin Virol Journal subject: Virology Year: 2022 Document Type: Article Affiliation country: J.jcv.2022.105318

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Adenoviruses, Human / Conjunctivitis / COVID-19 Type of study: Cohort study / Etiology study / Observational study / Prognostic study Limits: Humans Country/Region as subject: Asia Language: English Journal: J Clin Virol Journal subject: Virology Year: 2022 Document Type: Article Affiliation country: J.jcv.2022.105318