CRISPR-Cas-mediated diagnostics.
Trends Biotechnol
; 40(11): 1326-1345, 2022 Nov.
Article
in English
| MEDLINE | ID: covidwho-2061924
ABSTRACT
An ideal molecular diagnostic method should be sensitive, specific, low cost, rapid, portable, and easy to operate. Traditional nucleic acid detection methods based mainly on PCR technology have not only high sensitivity and specificity, but also some limitations, such as the need for expensive equipment and skilled technicians, being both time and labor intensive, and difficult to implement in some regions. However, with the continuous development of CRISPR-Cas technology and its application in molecular diagnosis, new approaches have been used for the construction of molecular diagnostic systems. In this review, we discuss recent advances in CRISPR-based molecular diagnostic technologies and highlight the revolution they bring to the field of molecular diagnostics.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Nucleic Acids
/
Gene Editing
Type of study:
Diagnostic study
Language:
English
Journal:
Trends Biotechnol
Year:
2022
Document Type:
Article
Affiliation country:
J.tibtech.2022.04.006
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