Your browser doesn't support javascript.
Probing Molecular Interactions of Antibody Drugs, Silicone Oil, and Surfactant at Buried Interfaces In Situ.
Lu, Tieyi; Fu, Li; Qiu, Yu; Zhang, Jifeng; Chen, Zhan.
  • Lu T; Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, United States.
  • Fu L; Sanofi, 1 The Mountain Road, Framingham, Massachusetts 01701, United States.
  • Qiu Y; Sanofi, 350 Water St, Cambridge, Massachusetts 02141, United States.
  • Zhang J; Sanofi, 1 The Mountain Road, Framingham, Massachusetts 01701, United States.
  • Chen Z; Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, United States.
Anal Chem ; 94(42): 14761-14768, 2022 10 25.
Article in English | MEDLINE | ID: covidwho-2062140
ABSTRACT
Antibody drugs have been rapidly developed to cure many diseases including COVID-19 infection. Silicone oil is commonly used as a lubricant coating material for devices used in the pharmaceutical industry to store and administer antibody drug formulations. However, the interaction between silicone oil and antibody molecules could lead to the adsorption, denaturation, and aggregation of antibody molecules, impacting the efficacy of antibody drugs. Here, we studied the molecular interactions between antibodies and silicone oil in situ in real time. The effect of the surfactant on such interactions was also investigated. Specifically, the adsorption dynamics of a bispecific antibody (BsAb) onto a silicone oil surface without and with different concentrations of the surfactant PS80 in antibody solutions were monitored. Also the possible lowest effective PS80 concentrations that can prevent the adsorption of BsAb as well as a monoclonal antibody (mAb) onto silicone oil were measured. It was found that different concentrations of PS80 are required for preventing the adsorption of different antibodies. Both BsAB and mAB denature on silicone oil without a surfactant. However, for a low surfactant concentration in the solution, although the surfactant could not completely prevent the antibody from adsorption, it could maintain the native structures of adsorbed BsAb and mAb antibodies on silicone oil. This is important knowledge, showing that to prevent antibody aggregation on silicone oil it is not necessary to add surfactant to a concentration high enough to completely minimize protein adsorption.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Bispecific / COVID-19 Limits: Humans Language: English Journal: Anal Chem Year: 2022 Document Type: Article Affiliation country: Acs.analchem.2c03425

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Bispecific / COVID-19 Limits: Humans Language: English Journal: Anal Chem Year: 2022 Document Type: Article Affiliation country: Acs.analchem.2c03425