Metagenomic Next-Generation Sequencing Reveals the Profile of Viral Infections in Kidney Transplant Recipients During the Covid-19 Pandemic
American Journal of Transplantation
; 22(Supplement 3):572, 2022.
Article
in English
| EMBASE | ID: covidwho-2063393
ABSTRACT
Purpose:
To study the clinical application of metagenomic next-generation sequencing (mNGS) in the detection of viral infections in kidney transplant recipients (KTRs) during the COVID-19 pandemic. Method(s) Using mNGS techniques, 50 human fluid samples of KTRs were detected in Henan Province People's Hospital between May 2020 to May 2021, including 20 bronchoalveolar lavage fluid (BALF) samples, 21 urine samples and 9 blood samples. The detected nucleic acid sequences were compared and analyzed with the existing viral nucleic acid sequences in the database, and the virus infection spectrum of KTRs was drawn. Result(s) The viral nucleic acids of 15 types of viruses were detected in 96.00% (48/50) of the samples, of which 11 types of viruses were in BALF (95.00%, 19/20), and the dominant viruses were torque teno virus (TTV) (65.00%;13/20), cytomegalovirus (CMV) (45.00%;9/20) and human alphaherpesvirus 1 (25.00%;5/20). 12 viruses (95.24%, 20/21) were detected in the urine, and the dominant viruses were TTV (52.38%;11/21), JC polyomavirus (52.38%;11/21), BK polyomavirus (42.86%;9/21), CMV (33.33%;7/21) and human betaherpesvirus 6B (28.57%;6/21). 7 viruses were detected in the blood (100.00%, 9/9), and the dominant virus was TTV (100.00%;9/9). Four rare viruses were detected in BALF and urine, including WU polyomavirus, primate bocaparvovirus 1, simian virus 12, and volepox virus. Further analysis showed that TTV infection with high reads indicated a higher risk of acute rejection (P<0.05). Conclusion(s) mNGS detection reveals the rich virus spectrum of infected persons after kidney transplantation, and improves the detection rate of rare viruses. TTV may be a new biomarker for predicting rejection. (Figure Presented).
acute graft rejection; adult; BK virus; blood sampling; Bocaparvovirus; bronchoalveolar lavage fluid; clinical article; conference abstract; controlled study; coronavirus disease 2019; Cytomegalovirus; female; high throughput sequencing; human; Human alphaherpesvirus 1; Human herpesvirus 6B; human tissue; JC virus; kidney graft; kidney transplantation; male; metagenomics; nonhuman; nucleotide sequence; pandemic; Polyomavirus; primate; simian virus; surgery; Torque teno virus infection; urine sampling; biological marker; nucleic acid
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
American Journal of Transplantation
Year:
2022
Document Type:
Article
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