Higher Proinflammatory Cytokines Are Associated with Increased Antibody Titer After a Third Dose of SARS-CoV-2 Vaccine in Solid Organ Transplant Recipients
American Journal of Transplantation
; 22(Supplement 3):637-638, 2022.
Article
in English
| EMBASE | ID: covidwho-2063471
ABSTRACT
Purpose:
Solid organ transplant recipients (SOTRs) are at increased risk for severe COVID-19 and exhibit lower antibody responses to SARS-CoV-2 vaccines. This study aimed to determine if pre-vaccination cytokine levels are associated with antibody response to SARS-CoV-2 vaccination. Method(s) A cross-sectional study was performed among 58 SOTRs before and after two-dose mRNA vaccine series, 35 additional SOTRs before and after a third vaccine dose, with comparison to 16 healthy controls (HCs). Anti-spike antibody was assessed using the IgG Euroimmun ELISA. Electrochemiluminescence detectionbased multiplexed sandwich immunoassays were used to quantify plasma cytokine and chemokine concentrations (n=20 analytes). Concentrations between SOTRs and HCs, stratified by ultimate antibody response to the vaccine, were compared using Wilcoxon-rank-sum test with false discovery rates (FDR) computed to correct for multiple comparisons. Result(s) In the study population, 100% of HCs, 59% of SOTRs after two doses and 63% of SOTRs after three doses had a detectable antibody response. Multiple baseline cytokines were elevated in SOTRs versus HCs. There was no significant difference in cytokine levels between SOTRs with high vs low-titer antibodies after two doses of vaccine. However, as compared to poor antibody responders, SOTRs who went on to develop a high-titer antibody response to a third dose of vaccine had significantly higher pre-third dose levels of several innate immune cytokines including IL-17, IL-2Ra, IL-6, IP-10, MIP-1alpha, and TNF-alpha (FDR <0.05). Conclusion(s) A specific inflammatory profile or immune state may identify which SOTRs are likely to develop stronger sero-response and possible protection after a third dose of SARS-CoV-2 vaccine.
adult; antibody response; antibody titer; conference abstract; controlled study; cross-sectional study; drug megadose; drug therapy; electrochemiluminescence; enzyme linked immunosorbent assay; false discovery rate; female; gene expression; graft recipient; human; human tissue; immunoassay; major clinical study; male; nonhuman; protein blood level; rank sum test; Severe acute respiratory syndrome coronavirus 2; spike; vaccination; chemokine; cytokine; endogenous compound; gamma interferon inducible protein 10; immunoglobulin G; interleukin 17; interleukin 2 receptor alpha; interleukin 6; macrophage inflammatory protein 1alpha; RNA vaccine; SARS-CoV-2 vaccine; tumor necrosis factor
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Topics:
Vaccines
Language:
English
Journal:
American Journal of Transplantation
Year:
2022
Document Type:
Article
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