Precision-cut lung slices: A powerful ex vivo model to investigate respiratory infectious diseases.
Mol Microbiol
; 117(3): 578-588, 2022 03.
Article
in English
| MEDLINE | ID: covidwho-2063875
ABSTRACT
Respiratory infections are a leading cause of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid, or surrogate animal models. Although these provide important insights, they have limitations. Cell culture models fail to recapitulate cellular interactions in the lung and animal models often do not permit high-throughput analysis of drugs or pathogen isolates; hence, there is a need for improved, scalable models. Precision-cut lung slices (PCLS), small, uniform tissue slices generated from animal or human lungs are increasingly recognized and employed as an ex vivo organotypic model. PCLS retain remarkable cellular complexity and the architecture of the lung, providing a platform to investigate respiratory pathogens in a near-native environment. Here, we review the generation and features of PCLS, their use to investigate the pathogenesis of viral and bacterial pathogens, and highlight their potential to advance respiratory infection research in the future.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Communicable Diseases
/
Lung
Limits:
Animals
Language:
English
Journal:
Mol Microbiol
Journal subject:
Molecular Biology
/
Microbiology
Year:
2022
Document Type:
Article
Affiliation country:
Mmi.14817
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