Your browser doesn't support javascript.
Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016-2017.
Dong, Hieu Van; Suzuki, Maya; Takemae, Hitoshi; Jamsransuren, Dulamjav; Matsuda, Sachiko; Nguyen, Hiep Dinh; Mizutani, Tetsuya; Takeda, Yohei; Ogawa, Haruko.
  • Dong HV; Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Suzuki M; Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam.
  • Takemae H; Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Jamsransuren D; Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
  • Matsuda S; Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Nguyen HD; Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Mizutani T; Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Takeda Y; Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
  • Ogawa H; Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
J Vet Med Sci ; 84(11): 1543-1550, 2022 Nov 14.
Article in English | MEDLINE | ID: covidwho-2065087
ABSTRACT
In this study, the viral genome extraction performance of automatic nucleic acid extractors and manual nucleic acid extraction kits was compared. We showed that compared with manual kits, the automatic extractors showed superior genome extraction performance using bovine viral diarrhea virus (BVDV) genome-positive cattle sera and bovine coronavirus/infectious bovine rhinotracheitis virus-spiked cattle nasal swabs. In addition, the subgenotyping of BVDV strains detected in Tokachi Province in Japan during 2016-2017 was performed. Results showed that most of these BVDV strains belonged to subgenotype 1b, while few strains belonged to subgenotypes 1a and 2a. This study showed the high applicability of automatic nucleic acid extractors in extracting multiple viral genomes and the dominant subgenotype of BVDV in Tokachi.
Subject(s)
Keywords
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: Bovine Virus Diarrhea-Mucosal Disease / Nucleic Acids / Cattle Diseases / Diarrhea Viruses, Bovine Viral / Diarrhea Virus 1, Bovine Viral Type of study: Randomized controlled trials Limits: Animals Country/Region as subject: Asia Language: English Journal: J Vet Med Sci Journal subject: Veterinary Medicine Year: 2022 Document Type: Article Affiliation country: Jvms.22-0096

Similar

MEDLINE
LILACS
LIS
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: Bovine Virus Diarrhea-Mucosal Disease / Nucleic Acids / Cattle Diseases / Diarrhea Viruses, Bovine Viral / Diarrhea Virus 1, Bovine Viral Type of study: Randomized controlled trials Limits: Animals Country/Region as subject: Asia Language: English Journal: J Vet Med Sci Journal subject: Veterinary Medicine Year: 2022 Document Type: Article Affiliation country: Jvms.22-0096