Technologies Enabling Single-Molecule Super-Resolution Imaging of mRNA.
Cells
; 11(19)2022 09 30.
Article
in English
| MEDLINE | ID: covidwho-2065729
ABSTRACT
The transient nature of RNA has rendered it one of the more difficult biological targets for imaging. This difficulty stems both from the physical properties of RNA as well as the temporal constraints associated therewith. These concerns are further complicated by the difficulty in imaging endogenous RNA within a cell that has been transfected with a target sequence. These concerns, combined with traditional concerns associated with super-resolution light microscopy has made the imaging of this critical target difficult. Recent advances have provided researchers the tools to image endogenous RNA in live cells at both the cellular and single-molecule level. Here, we review techniques used for labeling and imaging RNA with special emphases on various labeling methods and a virtual 3D super-resolution imaging technique.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Imaging, Three-Dimensional
/
Single Molecule Imaging
Language:
English
Year:
2022
Document Type:
Article
Affiliation country:
Cells11193079
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