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t6A and ms2t6A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity.
Nagayoshi, Yu; Nishiguchi, Kayo; Yamamura, Ryosuke; Chujo, Takeshi; Oshiumi, Hiroyuki; Nagata, Hiroko; Kaneko, Hitomi; Yamamoto, Keiichi; Nakata, Hirotomo; Sakakida, Korin; Kunisawa, Akihiro; Adachi, Masataka; Kakizoe, Yutaka; Mizobe, Takanori; Kuratsu, Jun-Ichi; Shimada, Shinya; Nakamori, Yasushi; Matsuoka, Masao; Mukoyama, Masashi; Wei, Fan-Yan; Tomizawa, Kazuhito.
  • Nagayoshi Y; Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Nishiguchi K; Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Yamamura R; Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Chujo T; Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Oshiumi H; Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Nagata H; Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Kaneko H; Department of Immunology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Yamamoto K; Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Nakata H; Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Sakakida K; Department of Laboratory Medicine, Kumamoto University Hospital, Kumamoto 860-8556, Japan.
  • Kunisawa A; Department of Hematology, Rheumatology and Infectious Diseases, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Adachi M; Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Kakizoe Y; Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Mizobe T; Shimadzu Corporation, Kyoto 604-8442, Japan.
  • Kuratsu JI; Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Shimada S; Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Nakamori Y; Kumamoto Kenhoku Hospital, Kumamoto 865-8005, Japan.
  • Matsuoka M; Sakurajyuji Hospital, Kumamoto 861-4173, Japan.
  • Mukoyama M; JCHO Kumamoto General Hospital, Kumamoto 866-8660, Japan.
  • Wei FY; Department of Emergency and Critical Care Medicine, Kansai Medical University General Medical Center, Osaka 570-8507, Japan.
  • Tomizawa K; Department of Hematology, Rheumatology and Infectious Diseases, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
Biomolecules ; 12(9)2022 09 03.
Article in English | MEDLINE | ID: covidwho-2273506
ABSTRACT
SARS-CoV-2 infection alters cellular RNA content. Cellular RNAs are chemically modified and eventually degraded, depositing modified nucleosides into extracellular fluids such as serum and urine. Here we searched for COVID-19-specific changes in modified nucleoside levels contained in serum and urine of 308 COVID-19 patients using liquid chromatography-mass spectrometry (LC-MS). We found that two modified nucleosides, N6-threonylcarbamoyladenosine (t6A) and 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A), were elevated in serum and urine of COVID-19 patients. Moreover, these levels were associated with symptom severity and decreased upon recovery from COVID-19. In addition, the elevation of similarly modified nucleosides was observed regardless of COVID-19 variants. These findings illuminate specific modified RNA nucleosides in the extracellular fluids as biomarkers for COVID-19 infection and severity.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 / Nucleosides Type of study: Diagnostic study / Prognostic study Topics: Variants Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Biom12091233

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 / Nucleosides Type of study: Diagnostic study / Prognostic study Topics: Variants Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Biom12091233