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Assessing anti-SARS-CoV-2 cellular immunity in 571 vaccines by using an IFN-γ release assay.
Wakui, Masatoshi; Uwamino, Yoshifumi; Yatabe, Yoko; Nakagawa, Terumichi; Sakai, Akiko; Kurafuji, Toshinobu; Shibata, Ayako; Tomita, Yukari; Noguchi, Masayo; Tanabe, Akiko; Arai, Tomoko; Ohno, Akemi; Yokota, Hiromitsu; Uno, Shunsuke; Yamasawa, Wakako; Sato, Yasunori; Ikeda, Mari; Yoshimura, Akihiko; Hasegawa, Naoki; Saya, Hideyuki; Murata, Mitsuru.
  • Wakui M; Department of Laboratory Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Uwamino Y; Department of Laboratory Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Yatabe Y; Department of Infectious Diseases, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Nakagawa T; Clinical Laboratory, Keio University Hospital, Shinjuku-ku, Tokyo, Japan.
  • Sakai A; Clinical Laboratory, Keio University Hospital, Shinjuku-ku, Tokyo, Japan.
  • Kurafuji T; Clinical Laboratory, Keio University Hospital, Shinjuku-ku, Tokyo, Japan.
  • Shibata A; Clinical Laboratory, Keio University Hospital, Shinjuku-ku, Tokyo, Japan.
  • Tomita Y; Department of Laboratory Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Noguchi M; Department of Laboratory Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Tanabe A; Clinical Laboratory, Keio University Hospital, Shinjuku-ku, Tokyo, Japan.
  • Arai T; Clinical Laboratory, Keio University Hospital, Shinjuku-ku, Tokyo, Japan.
  • Ohno A; Clinical Laboratory, Keio University Hospital, Shinjuku-ku, Tokyo, Japan.
  • Yokota H; Clinical Laboratory, Keio University Hospital, Shinjuku-ku, Tokyo, Japan.
  • Uno S; Clinical Laboratory, Keio University Hospital, Shinjuku-ku, Tokyo, Japan.
  • Yamasawa W; Department of Infectious Diseases, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Sato Y; Department of Laboratory Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Ikeda M; Department of Epidemiology and Preventive Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Yoshimura A; Department of Microbiology and Immunology, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Hasegawa N; Department of Microbiology and Immunology, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Saya H; Department of Infectious Diseases, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
  • Murata M; Division of Gene Regulation, Institute for Advanced Medical Research, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
Eur J Immunol ; 2022 Oct 17.
Article in English | MEDLINE | ID: covidwho-2127680
ABSTRACT
Memory T cell responses have been analyzed only in small cohorts of COVID-19 vaccines. Herein, we aimed to assess anti-SARS-CoV-2 cellular immunity in a large cohort using QuantiFERON assays, which are IFN-γ release assays (IGRAs) based on short-term whole blood culture. The study included 571 individuals receiving the viral spike (S) protein-expressing BNT162b2 mRNA vaccine. QuantiFERON assays revealed antigen-specific IFN-γ production in most individuals 8 weeks after the second dose. Simultaneous flow cytometric assays to detect T cells expressing activation-induced markers (AIMs) performed for 28 randomly selected individuals provided data correlating with the QuantiFERON data. Simultaneous IFN-γ enzyme-linked immunospot and AIM assays for another subset of 31 individuals, based on short-term peripheral blood mononuclear cell culture, also indicated a correlation between IFN-γ production and AIM positivity. These observations indicated the acquisition of T cell memory responses and supported the usability of IGRAs to assess cellular immunity. The QuantiFERON results were weakly correlated with serum IgG titers against the receptor-binding domain of the S protein and were associated with pre-vaccination infection and adverse reactions after the second dose. The present study revealed cellular immunity after COVID-19 vaccination, providing insights into the effects and adverse reactions of vaccination.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Topics: Vaccines Language: English Year: 2022 Document Type: Article Affiliation country: Eji.202249794

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Topics: Vaccines Language: English Year: 2022 Document Type: Article Affiliation country: Eji.202249794