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Comparison of nucleocapsid and spike antibody ELISAs for determining SARS-CoV-2 seropositivity in Kenyan women and infants.
Fish, Carolyn S; Owiti, Prestone; Begnel, Emily R; Itell, Hannah L; Ojee, Ednah; Adhiambo, Judith; Ogweno, Vincent; Holland, LaRinda A; Richardson, Barbra A; Khan, Adam K; Maqsood, Rabia; Gantt, Soren; Lim, Efrem S; Slyker, Jennifer; Kinuthia, John; Overbaugh, Julie; Wamalwa, Dalton; Lehman, Dara A; Chohan, Bhavna H.
  • Fish CS; Division of Human Biology, Fred Hutchinson Cancer Center, Seattle, Washington, USA.
  • Owiti P; Department of Paediatrics and Child Health, University of Nairobi, Nairobi, Kenya.
  • Begnel ER; Department of Global Health, Hans Rosling Center, University of Washington, Seattle, Washington, USA.
  • Itell HL; Division of Human Biology, Fred Hutchinson Cancer Center, Seattle, Washington, USA.
  • Ojee E; Molecular and Cellular Biology Graduate Program, University of Washington and Fred Hutchinson Cancer Center, Seattle, Washington, USA.
  • Adhiambo J; Department of Paediatrics and Child Health, University of Nairobi, Nairobi, Kenya.
  • Ogweno V; Department of Paediatrics and Child Health, University of Nairobi, Nairobi, Kenya.
  • Holland LA; Department of Paediatrics and Child Health, University of Nairobi, Nairobi, Kenya.
  • Richardson BA; Center for Fundamental and Applied Microbiomics, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Khan AK; Department of Global Health, Hans Rosling Center, University of Washington, Seattle, Washington, USA.
  • Maqsood R; Department of Biostatistics, University of Washington, Seattle, Washington, USA.
  • Gantt S; School of Life Sciences, Arizona State University, Tempe, Arizona, USA.
  • Lim ES; Center for Fundamental and Applied Microbiomics, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Slyker J; Département de Microbiologie, Infectiologie et Immunologie, Centre de Recherche du CHU St-Justine, Université de Montréal, Montréal, Québec, Canada.
  • Kinuthia J; Center for Fundamental and Applied Microbiomics, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
  • Overbaugh J; School of Life Sciences, Arizona State University, Tempe, Arizona, USA.
  • Wamalwa D; Department of Global Health, Hans Rosling Center, University of Washington, Seattle, Washington, USA.
  • Lehman DA; Department of Epidemiology, University of Washington, Seattle, Washington, USA.
  • Chohan BH; Department of Global Health, Hans Rosling Center, University of Washington, Seattle, Washington, USA.
J Med Virol ; 2022 Oct 17.
Article in English | MEDLINE | ID: covidwho-2230017
ABSTRACT
A multitude of enzyme-linked immunosorbent assays (ELISAs) has been developed to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies since the coronavirus disease 2019 pandemic started in late 2019. Assessing the reliability of these assays in diverse global populations is critical. This study compares the use of the commercially available Platelia Total Ab Assay (Bio-Rad) nucleocapsid ELISA to the widely used Mount Sinai spike IgG ELISA in a Kenyan population seroprevalence study. Using longitudinal plasma specimens collected from a mother-infant cohort living in Nairobi, Kenya between May 2019 and December 2020, this study demonstrates that the two assays have a high qualitative agreement (92.7%) and strong correlation of antibody levels (R2 = 0.973) in repeated measures. Within this cohort, seroprevalence detected by either ELISA closely resembled previously published seroprevalence estimates for Kenya during the sampling period and no significant difference in the incidence of SARS-CoV-2 antibody detection by either assay was observed. Assay comparability was not affected by HIV exposure status. These data support the use of the Platelia SARS-CoV-2 Total Ab ELISA as a suitable high-throughput method for seroprevalence studies in Kenya.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Observational study / Prognostic study / Qualitative research Language: English Year: 2022 Document Type: Article Affiliation country: Jmv.28221

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Observational study / Prognostic study / Qualitative research Language: English Year: 2022 Document Type: Article Affiliation country: Jmv.28221