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Detection of SARS-CoV-2 Neutralizing Antibodies in Vaccinated Pregnant Women and Neonates by Using a Lateral Flow Immunoassay Coupled with a Spectrum-Based Reader.
Chen, Wei-Chun; Lin, Yen-Pin; Cheng, Chao-Min; Shen, Ching-Fen; Li, Chang-Wei; Wang, Yu-Kuo; Shih, Ting-Ying; Hong, Chitsung; Chang, Ting-Chang; Shen, Ching-Ju.
  • Chen WC; Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
  • Lin YP; Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital at Linkou, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan.
  • Cheng CM; Department of Obstetrics and Gynecology, New Taipei City Municipal Tucheng Hospital, New Taipei City 236, Taiwan.
  • Shen CF; Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
  • Li CW; Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
  • Wang YK; Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
  • Shih TY; AllBio Life Inc., Taichung 408, Taiwan.
  • Hong C; AllBio Life Inc., Taichung 408, Taiwan.
  • Chang TC; AllBio Life Inc., Taichung 408, Taiwan.
  • Shen CJ; Spectrochip Inc., Hsinchu 302, Taiwan.
Biosensors (Basel) ; 12(10)2022 Oct 18.
Article in English | MEDLINE | ID: covidwho-2081892
ABSTRACT
The focus of this study was to investigate the detection of neutralizing antibodies (Nabs) in maternal serum and cord blood as the targeted samples by employing a lateral flow immunoassay combined with a spectrum reader (LFI-SR) and the correlation of Nab protection against different types of SARS-CoV-2. We enrolled 20 pregnant women who were vaccinated with the Moderna (mRNA-1273) vaccine during pregnancy and collected 40 samples during delivery. We used an LFI-SR for the level of spike protein receptor binding domain antibody (SRBD IgG) as Nabs and examined the correlation of the SRBD IgG concentration and Nab inhibition rates (NabIR) via enzyme-linked immunosorbent assays (ELISA). The LFI-SR had high confidence for the SRBD IgG level (p < 0.0001). Better NabIR were found in wild-type SARS-CoV-2 (WT) compared to Delta-type (DT) and Omicron-type (OT). Women with two-dose vaccinations demonstrated greater NabIR than those with a single dose. The cut-off value of the SRBD IgG level by the LFI-SR for NabIR to DT (≥30%; ≥70%) was 60.15 and 150.21 ng/mL for mothers (both p = 0.005), and 156.31 (p = 0.011) and 230.20 ng/mL (p = 0.006) for babies, respectively. An additional vaccine booster may be considered for those mothers with SRBD IgG levels < 60.15 ng/mL, and close protection should be given for those neonates with SRBD IgG levels < 150.21 ng/mL, since there is no available vaccine for them.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Topics: Vaccines / Variants Limits: Female / Humans / Infant, Newborn / Pregnancy Language: English Year: 2022 Document Type: Article Affiliation country: Bios12100891

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Topics: Vaccines / Variants Limits: Female / Humans / Infant, Newborn / Pregnancy Language: English Year: 2022 Document Type: Article Affiliation country: Bios12100891