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Intranasal SARS-CoV-2 spike-based immunisation adjuvanted with polyethyleneimine elicits mucosal and systemic humoral responses in mice.
Deimel, Lachlan P; Liu, Xin; Gilbert-Jaramillo, Javier; Liu, Sai; James, William S; Sattentau, Quentin J.
  • Deimel LP; Sir William Dunn School of Pathology, The University of Oxford, Oxford OX1 3RE, UK; The Department of Chemistry, The University of Oxford, OX1 3TA, UK. Electronic address: Lachlan.Deimel@path.ox.ac.uk.
  • Liu X; Sir William Dunn School of Pathology, The University of Oxford, Oxford OX1 3RE, UK.
  • Gilbert-Jaramillo J; James and Lillian Martin Centre, Sir William Dunn School of Pathology, The University of Oxford, Oxford OX1 3RE, UK.
  • Liu S; James and Lillian Martin Centre, Sir William Dunn School of Pathology, The University of Oxford, Oxford OX1 3RE, UK.
  • James WS; James and Lillian Martin Centre, Sir William Dunn School of Pathology, The University of Oxford, Oxford OX1 3RE, UK.
  • Sattentau QJ; Sir William Dunn School of Pathology, The University of Oxford, Oxford OX1 3RE, UK.
J Immunol Methods ; 511: 113380, 2022 Dec.
Article in English | MEDLINE | ID: covidwho-2086445
ABSTRACT
The SARS-CoV-2 pandemic continues despite the presence of effective vaccines, and novel vaccine approaches may help to reduce viral spread and associated COVID-19 disease. Current vaccine administration modalities are based on systemic needle-administered immunisation which may be suboptimal for mucosal pathogens. Here we demonstrate in a mouse model that small-volume intranasal administration of purified spike (S) protein in the adjuvant polyethylenemine (PEI) elicits robust antibody responses with modest systemic neutralisation activity. Further, we test a heterologous intranasal immunisation regimen, priming with S and boosting with RBD-Fc. Our data identify small volume PEI adjuvantation as a novel platform with potential for protective mucosal vaccine development.
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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Journal: J Immunol Methods Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Journal: J Immunol Methods Year: 2022 Document Type: Article