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An integrative analysis of Qingfei Paidu Decoction for its anti-HCoV-229E mechanism in cold and damp environment based on the pharmacokinetics, metabolomics and molecular docking technology.
Zhang, Yan; Gu, Xinru; Zhou, Yanyan; Si, Nan; Gao, Wenya; Sun, Bo; Sun, Jing; Li, Tao; Wang, Linna; Wei, Xiaolu; Guo, Shanshan; Cui, Xiaolan; Bian, Baolin; Wang, Hongjie; Wang, Liang; Zhao, Haiyu.
  • Zhang Y; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China. Electronic address: 1784865127@qq.com.
  • Gu X; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Zhou Y; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Si N; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Gao W; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Sun B; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Sun J; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Li T; China Academy of Chinese Medical Sciences, Medical Experimental Center, Beijing 100007, China.
  • Wang L; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Wei X; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Guo S; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Cui X; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Bian B; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China.
  • Wang H; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China. Electronic address: hjwang@icmm.ac.cn.
  • Wang L; China Resources Sanjiu Medical and Pharmaceutical Co., Ltd., Shenzhen 518110, China. Electronic address: wangliang@999.com.cn.
  • Zhao H; China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100007, China. Electronic address: hyzhao@icmm.ac.cn.
Phytomedicine ; 108: 154527, 2023 Jan.
Article in English | MEDLINE | ID: covidwho-2086623
ABSTRACT

BACKGROUND:

The novel coronavirus pneumonia (COVID-19) has spread rapidly around the world. As a member against the epidemic, Qingfei Paidu Decoction (QFPDD) has been approved for the treatment of COVID-19 in China. However, its antiviral mechanism was still largely unclear.

PURPOSE:

An integrated strategy was used to explore the antiviral mechanisms of QFPDD in cold and damp environment, including pharmacokinetic (PK), network pharmacology, metabolomics and protein verification.

METHODS:

Firstly, the pharmacokinetic study of the prototype absorbed ingredients were analyzed by UHPLC-QqQ-MS. Secondly, the metabolomics analysis of the endogenous constituents was carried out. Based on the aforementioned results, an integrated network was constructed to identify the curative components, crucial endogenous differential metabolites and related pathways. Finally, the validation tests were implemented by molecular docking and western blotting (WB).

RESULTS:

According to the pharmacokinetic behaviors analysis of 31 components in vivo, the flavonoids presented more longer residence time and higher exposure compared with the other compounds. The efficacy and antiviral mechanism of QFPDD were verified by the poly-pharmacology, metabolomics, molecular docking and WB. For the occurrence of metabolic disorder, the change of amino acid transporters should not be neglected. Afterward, 8 curative compounds, 6 key genes and corresponding metabolic pathways were filtered by compound-reaction-enzyme-gene network. The molecular docking verified that the active ingredients bound to the relevant targets well.

CONCLUSION:

In the present study, an in vivo comprehensive pharmacokinetic behaviors of QFPDD was analyzed for the first time. The results illustrated that QFPDD could exhibit immune regulation, anti-infection, anti-inflammation and metabolic disorder to perform a corresponding therapeutic effect. Moreover, our findings highlighted the roles of amino acid transporters in the coronavirus infection situation.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Drugs, Chinese Herbal / Coronavirus 229E, Human / COVID-19 Drug Treatment Type of study: Prognostic study Topics: Traditional medicine Limits: Humans Language: English Journal: Phytomedicine Journal subject: Complementary Therapies Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Drugs, Chinese Herbal / Coronavirus 229E, Human / COVID-19 Drug Treatment Type of study: Prognostic study Topics: Traditional medicine Limits: Humans Language: English Journal: Phytomedicine Journal subject: Complementary Therapies Year: 2023 Document Type: Article