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MnO2 Nanozyme-Mediated CRISPR-Cas12a System for the Detection of SARS-CoV-2.
Wu, Lina; Wang, Xinjie; Wu, Xiangchuan; Xu, Shiqi; Liu, Ming; Cao, Xizhong; Tang, Taishan; Huang, Xingxu; Huang, He.
  • Wu L; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, People's Republic of China.
  • Wang X; Zhejiang Laboratory, Hangzhou 311100, People's Republic of China.
  • Wu X; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, People's Republic of China.
  • Xu S; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, People's Republic of China.
  • Liu M; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, People's Republic of China.
  • Cao X; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
  • Tang T; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
  • Huang X; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
  • Huang H; Zhejiang Laboratory, Hangzhou 311100, People's Republic of China.
ACS Appl Mater Interfaces ; 14(45): 50534-50542, 2022 Nov 16.
Article in English | MEDLINE | ID: covidwho-2087120
ABSTRACT
The CRISPR-Cas system was developed into a molecular diagnostic tool with high sensitivity, low cost, and high specificity in recent years. Colorimetric assays based on nanozymes offer an attractive point-of-care testing method for their low cost of use and user-friendly operation. Here, a MnO2 nanozyme-mediated CRISPR-Cas12a system was instituted to detect SARS-CoV-2. MnO2 nanorods linked to magnetic beads via a single-stranded DNA (ssDNA) linker used as an oxidase-like nanozyme inducing the color change of 3,3',5,5'-tetramethylbenzidine, which can be distinguished by the naked eye. The detection buffer color will change when the Cas12a is activated by SARS-CoV-2 and indiscriminately cleave the linker ssDNA. The detection limit was 10 copies per microliter and showed no cross-reaction with other coronaviruses. The nanozyme-mediated CRISPR-Cas12a system shows high selectivity and facile operation, with great potential for molecular diagnosis in point-of-care testing applications.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Randomized controlled trials Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Randomized controlled trials Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2022 Document Type: Article