Recent advances in PCR-free nucleic acid detection for SARS-COV-2.
Front Bioeng Biotechnol
; 10: 999358, 2022.
Article
in English
| MEDLINE | ID: covidwho-2089816
ABSTRACT
As the outbreak of Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory disease coronavirus 2 (SARS-COV-2), fast, accurate, and economic detection of viral infection has become crucial for stopping the spread. Polymerase chain reaction (PCR) of viral nucleic acids has been the gold standard method for SARS-COV-2 detection, which, however, generally requires sophisticated facilities and laboratory space, and is time consuming. This review presents recent advances in PCR-free nucleic acid detection methods for SARS-CoV-2, including emerging methods of isothermal amplification, nucleic acid enzymes, electrochemistry and CRISPR.
Full text:
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Collection:
International databases
Database:
MEDLINE
Language:
English
Journal:
Front Bioeng Biotechnol
Year:
2022
Document Type:
Article
Affiliation country:
Fbioe.2022.999358
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