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Microarray Profiling of Vaccination-Induced Antibody Responses to SARS-CoV-2 Variants of Interest and Concern.
Svetlova, Julia; Gustin, Dmitry; Manuvera, Valentin; Shirokov, Dmitriy; Shokina, Varvara; Prusakov, Kirill; Aldarov, Konstantin; Kharlampieva, Daria; Matyushkina, Daria; Bespyatykh, Julia; Varizhuk, Anna; Lazarev, Vassili; Vedekhina, Tatiana.
  • Svetlova J; Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia.
  • Gustin D; Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, Vernadsky Avenue, 86, 119454 Moscow, Russia.
  • Manuvera V; Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia.
  • Shirokov D; Moscow Institute of Physics and Technology (State University), 9, Institutskiy per., Moscow Region, 141701 Dolgoprudny, Russia.
  • Shokina V; Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia.
  • Prusakov K; K. I. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Akademika Skryabina Street, 23, 109472 Moscow, Russia.
  • Aldarov K; Scientific Research Institute for Systems Biology and Medicine, Scientific Driveway, 18, 117246 Moscow, Russia.
  • Kharlampieva D; Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia.
  • Matyushkina D; Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia.
  • Bespyatykh J; Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia.
  • Varizhuk A; Scientific Research Institute for Systems Biology and Medicine, Scientific Driveway, 18, 117246 Moscow, Russia.
  • Lazarev V; Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia.
  • Vedekhina T; Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia.
Int J Mol Sci ; 23(21)2022 Oct 30.
Article in English | MEDLINE | ID: covidwho-2090212
ABSTRACT
Mutations in surface proteins enable emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to escape a substantial fraction of neutralizing antibodies and may thus weaken vaccine-driven immunity. To compare available vaccines and justify revaccination, rapid evaluation of antibody (Ab) responses to currently circulating SARS-CoV-2 variants of interest (VOI) and concern (VOC) is needed. Here, we developed a multiplex protein microarray-based system for rapid profiling of anti-SARS-CoV-2 Ab levels in human sera. The microarray system was validated using sera samples from SARS-CoV-2-free donors and those diagnosed with COVID-19 based on PCR and enzyme immunoassays. Microarray-based profiling of vaccinated donors revealed a substantial difference in anti-VOC Ab levels elicited by the replication-deficient adenovirus vector-base (Sputnik V) and whole-virion (CoviVac Russia COVID-19) vaccines. Whole-virion vaccine-induced Abs showed minor but statistically significant cross-reactivity with the human blood coagulation factor 1 (fibrinogen) and thrombin. However, their effects on blood clotting were negligible, according to thrombin time tests, providing evidence against the concept of pronounced cross-reactivity-related side effects of the vaccine. Importantly, all samples were collected in the pre-Omicron period but showed noticeable responses to the receptor-binding domain (RBD) of the Omicron spike protein. Thus, using the new express Ab-profiling system, we confirmed the inter-variant cross-reactivity of the anti-SARS-CoV-2 Abs and demonstrated the relative potency of the vaccines against new VOCs.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Vaccines / Antibody Formation Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Topics: Vaccines / Variants Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Ijms232113220

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Vaccines / Antibody Formation Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Topics: Vaccines / Variants Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Ijms232113220