SARS-CoV-2 SNV Genotyping Test Technology

ABSTRACT

The global pandemic of the COVID-19 has had a major impact on the entire human society, and human beings are facing challenges such as fiscal stimulus, financial stress, and debt restructuring. Before the emergence of specific therapeutic drugs and methods, large-scale population screening and isolation has become the most effective method for epidemic management. However, the new strain of coronavirus this time has shown a very high genetic variability, with a statistical mutation rate of more than 2. 3%c as of March 31st, 2022. So far, new highly infectious virus strains have been emerging, and the number of mutant strains officially warned by the World Health Organization has reached 7. Therefore, in the next virus prevention and control and research, we not only need to detect SARS-CoV-2, but also need to explore accurate and practical single nucleotide variation (SNV) genotyping techniques, especially for large-scale population screening. It is not only necessary to obtain information on the SRAS-CoV-2, but also to accurately and quickly distinguish variant strains with higher infectivity and virulence. This paper briefly introduces the infection and mutation mechanism of the virus, and focuses on the classification and review of the existing main SARS-CoV-2 SNV genotyping techniques, hoping to provide insight into the development of new detection technolooies. © 2022, China Biotechnology Press. All rights reserved.