Experimental Protocol for Detecting SARS-CoV-2 in Screenings and Grit Samples of Wastewater Treatment Plants
Journal of Environmental Health and Sustainable Development
; 7(3):1727-1732, 2022.
Article
in English
| Scopus | ID: covidwho-2091178
ABSTRACT
Introduction:
Although various liquid, solid, and gaseous streams of wastewater treatment plants (WWTPs) have been analyzed in many studies for the presence of SARS-CoV-2 RNA, no study was found to sample and detect SARS-CoV-2 RNA in screenings and grit samples separated from primary treatment units of WWTP. Hence, this study aims to provide an experimental protocol for sampling and extracting SARS-CoV-2 RNA from screenings and grits separated from WWTPs. Materials andMethods:
First, sampling was conducted to extract SARS-CoV-2 RNA from screenings and grit samples. After sample processing and viral RNA extraction, SARS-CoV-2 RNA detection was performed by one-step reverse transcription quantitative polymerase chain reaction (RT-qPCR).Results:
Based on the results, SARS-CoV-2 RNA was successfully extracted from screenings and grit samples of the studied WWTP with concentrations of (1.54 –3.9 × 104) and (0.8 – 2.3 × 104) genomic copies/L, respectively.Conclusion:
Considering the successfully isolation and detection of SARSCoV-2 viral RNA in solid phase samples of WWTP, this method can be applied for extracting SARS-CoV-2 RNA and maybe other viruses from the screenings and grit samples of WWTPs in related studies © 2022, Journal of Environmental Health and Sustainable Development.All Rights Reserved.
Full text:
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Collection:
Databases of international organizations
Database:
Scopus
Language:
English
Journal:
Journal of Environmental Health and Sustainable Development
Year:
2022
Document Type:
Article
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