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Dynamics of peripheral immune cells and their HLA-G and receptor expressions in a patient suffering from critical COVID-19 pneumonia to convalescence.
Zhang, Sheng; Gan, Jun; Chen, Bao-Guo; Zheng, Dan; Zhang, Jian-Gang; Lin, Rong-Hai; Zhou, Yi-Ping; Yang, Wei-Ying; Lin, Aifen; Yan, Wei-Hua.
  • Zhang S; Department of Intensive Care Unit TaiZhou Hospital of Zhejiang Province Wenzhou Medical University LinHai Zhejiang China.
  • Gan J; Medical Research Center TaiZhou Hospital of Zhejiang Province Wenzhou Medical University LinHai Zhejiang China.
  • Chen BG; Medical Research Center TaiZhou Hospital of Zhejiang Province Wenzhou Medical University LinHai Zhejiang China.
  • Zheng D; Department of Intensive Care Unit TaiZhou Hospital of Zhejiang Province Wenzhou Medical University LinHai Zhejiang China.
  • Zhang JG; Biological Resource Center TaiZhou Hospital of Zhejiang Province Wenzhou Medical University LinHai Zhejiang China.
  • Lin RH; Department of Intensive Care Unit TaiZhou Hospital of Zhejiang Province Wenzhou Medical University LinHai Zhejiang China.
  • Zhou YP; Department of Intensive Care Unit TaiZhou Hospital of Zhejiang Province Wenzhou Medical University LinHai Zhejiang China.
  • Yang WY; Department of Intensive Care Unit TaiZhou Hospital of Zhejiang Province Wenzhou Medical University LinHai Zhejiang China.
  • Lin A; Biological Resource Center TaiZhou Hospital of Zhejiang Province Wenzhou Medical University LinHai Zhejiang China.
  • Yan WH; Medical Research Center TaiZhou Hospital of Zhejiang Province Wenzhou Medical University LinHai Zhejiang China.
Clin Transl Immunology ; 9(5): e1128, 2020 May.
Article in English | MEDLINE | ID: covidwho-209400
ABSTRACT

OBJECTIVES:

Host immune responses are indispensable to combat the disease. We report the dynamics of peripheral immune cells, cytokines, and human leucocyte antigen-G (HLA-G) and its receptor expressions in a patient suffering from critical COVID-19 pneumonia to convalescence.

METHODS:

Clinical data of the patient were collected from medical records. The expressions of HLA-G and receptors ILT2, ILT4 and KIR2DL4 in peripheral immune cells were measured with flow cytometry.

RESULTS:

From critical COVID-19 to the convalescent stage, early lymphopenia was improved (median 0.6 × 109 L-1 vs. 0.9 × 109 L-1, P = 0.009), and an obvious fluctuation in WBC and neutrophil counts was observed. Initially, low levels of CD4+ T cells (from 120 to 528 µL-1) and CD8+ T cells (from 68 to 362 µL-1) gradually increased to normal levels. Meanwhile, high IL-6 (from 251.8 to 6.32 pg mL-1), IL-10 (from 39.53 to 5.21 pg mL-1) and IFN-γ (from 13.55 to 3.16 pg mL-1) levels decreased, and IL-4 (from 2.36 to 3.19 pg mL-1) and TNF-α (from 2.27 to 20.2 pg mL-1) levels increased quickly when the viral RNA returned negative. Moreover, the percentage of HLA-G+ T cells, B cells and monocytes follows high-low-high pattern, while the percentage of receptors ILT2-, ILT4- and KIR2DL4-expressing cells remained relatively stable.

CONCLUSION:

Our findings provide valuable information on the dynamics of early peripheral immunological responses in SARS-CoV-2 infection. CD4+ and CD8+ T cells, cytokines and HLA-G+ immune cells are associated with the natural history of the critical COVID-19 patient; however, future studies are necessary.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Type of study: Prognostic study Language: English Journal: Clin Transl Immunology Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Prognostic study Language: English Journal: Clin Transl Immunology Year: 2020 Document Type: Article