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THE EXPRESSION LEVELS OF SARS-COV-2 INFECTIONMEDIATING MOLECULES PROMOTED BY INTERFERON-c AND TUMOR NECROSIS FACTOR-a ARE DOWNREGULATED BY HYDROGEN SULFIDE
Thyroid ; 32(Supplement 1):A47-A48, 2022.
Article in English | EMBASE | ID: covidwho-2097289
ABSTRACT

OBJECTIVE:

Angiotensin-converting enzyme 2 (ACE2) and neuropilin 1 (NRP1) were essential molecules mediating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The effects of typical cytokines in autoimmune thyroid diseases (AITDs), such as IFN-gamma, TNF-alpha, IL-4 on the expression of SARSCoV-2 infection-mediating molecules remained unclear. Hydrogen sulfide (H2S) had the effects of anti-virus in literatures. The purpose of our study was to explore whether IFN-gamma, TNF-alpha or IL-4 regulated ACE2 and NRP1 levels and whether H2S exerted protective roles in thyroid on SARS-CoV-2 infection by regulating these molecules. METHOD(S) Thyroid sections from the patients with HT (n = 18), GD (n = 18) and normal thyroid tissues (n = 18) were collected. Immunohistochemistry was used to detect the expression of ACE2 and NRP1. Human primary thyrocytes were stimulated with different concentrations of IFN-gamma, TNF-alpha and IL-4, respectively. Thyrocytes were further cultured with IFN-gamma, TNF-alpha or NaHS + IFN-gamma / TNF-alpha in vitro. ACE2 and NRP1 expression levels were examined by realtime PCR and Western blot. RESULT(S) Both ACE2 and NRP1 were expressed on human thyrocytes. ACE2 expressions in thyroid tissues were higher in HT group than normal thyroid group (P < 0.001) and staining scores for ACE2 had no relationship with serum levels of thyroid autoantibodies. NRP1 expressions in HT and GD thyroid tissues were higher than those in normal thyroid tissues (P < 0.0001) and the staining scores for NRP1 were positively correlated with serum levels of TgAb (r = 0.4627, P = 0.0024) and TPOAb (r = 0.3633, P = 0.0181). The mRNA levels of ACE2 and NRP1 were upregulated in a concentration-dependent manner for IFN-gamma and TNF-alpha in human primary thyrocytes (P < 0.05). IL-4 rarely had effects on ACE2 or NRP1 mRNA levels. After pre-treating with NaHS, we found that ACE2 and NRP1 protein expressions were downregulated compared to IFN-gamma or TNF-alpha treatment (P < 0.01). CONCLUSION(S) ACE2 and NRP1 expressions were more abundant in thyroid tissues from AITDs than normal thyroid tissues. ACE2 and NRP1 expressions were upregulated by IFN-gamma and TNF-alpha. H2S might exert a protective role against SARS-CoV-2 infection by downregulating ACE2 and NRP1 levels.
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Full text: Available Collection: Databases of international organizations Database: EMBASE Language: English Journal: Thyroid Year: 2022 Document Type: Article

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Full text: Available Collection: Databases of international organizations Database: EMBASE Language: English Journal: Thyroid Year: 2022 Document Type: Article