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A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019.
Cai, Xue-Fei; Chen, Juan; Li Hu, Jie-; Long, Quan-Xin; Deng, Hai-Jun; Liu, Ping; Fan, Kai; Liao, Pu; Liu, Bei-Zhong; Wu, Gui-Cheng; Chen, Yao-Kai; Li, Zhi-Jie; Wang, Kun; Zhang, Xiao-Li; Tian, Wen-Guang; Xiang, Jiang-Lin; Du, Hong-Xin; Wang, Jing; Hu, Yuan; Tang, Ni; Lin, Yong; Ren, Ji-Hua; Huang, Lu-Yi; Wei, Jie; Gan, Chun-Yang; Chen, Yan-Meng; Gao, Qing-Zhu; Chen, A-Mei; He, Chang-Long; Wang, Dao-Xin; Hu, Peng; Zhou, Fa-Chun; Huang, Ai-Long; Wang, De-Qiang.
  • Cai XF; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Chen J; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Li Hu J; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Long QX; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Deng HJ; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Liu P; BioScience Co. LTD, Tianjin, China.
  • Fan K; PEG-Bio Biopharm Co. LTD., Chongqing, China.
  • Liao P; Laboratory Department, Chongqing People's Hospital, Chongqing, China.
  • Liu BZ; Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China.
  • Wu GC; Chongqing Three Gorges Central Hospital, Chongqing, China.
  • Chen YK; The Public Health Center, Chongqing, China.
  • Li ZJ; Laboratory Department, Chongqing People's Hospital, Chongqing, China.
  • Wang K; Laboratory Department, Chongqing People's Hospital, Chongqing, China.
  • Zhang XL; Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China.
  • Tian WG; Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China.
  • Xiang JL; Chongqing Three Gorges Central Hospital, Chongqing, China.
  • Du HX; Chongqing Three Gorges Central Hospital, Chongqing, China.
  • Wang J; The Public Health Center, Chongqing, China.
  • Hu Y; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Tang N; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Lin Y; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Ren JH; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Huang LY; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Wei J; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Gan CY; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Chen YM; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Gao QZ; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Chen AM; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • He CL; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Wang DX; Department of Respiration, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
  • Hu P; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Zhou FC; Department of Emergency, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
  • Huang AL; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Wang DQ; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
J Infect Dis ; 222(2): 189-193, 2020 06 29.
Article in English | MEDLINE | ID: covidwho-643587
ABSTRACT

BACKGROUND:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel ß-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection.

METHODS:

In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2.

RESULTS:

To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively.

CONCLUSIONS:

Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Serologic Tests / Immunoenzyme Techniques / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Prognostic study Topics: Vaccines Limits: Adult / Female / Humans / Male / Middle aged Language: English Journal: J Infect Dis Year: 2020 Document Type: Article Affiliation country: Infdis

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Serologic Tests / Immunoenzyme Techniques / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Prognostic study Topics: Vaccines Limits: Adult / Female / Humans / Male / Middle aged Language: English Journal: J Infect Dis Year: 2020 Document Type: Article Affiliation country: Infdis