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Anatomical barriers against SARS-CoV-2 neuroinvasion at vulnerable interfaces visualized in deceased COVID-19 patients.
Khan, Mona; Clijsters, Marnick; Choi, Sumin; Backaert, Wout; Claerhout, Michiel; Couvreur, Floor; Van Breda, Laure; Bourgeois, Florence; Speleman, Kato; Klein, Sam; Van Laethem, Johan; Verstappen, Gill; Dereli, Ayse Sumeyra; Yoo, Seung-Jun; Zhou, Hai; Dan Do, Thuc Nguyen; Jochmans, Dirk; Laenen, Lies; Debaveye, Yves; De Munter, Paul; Gunst, Jan; Jorissen, Mark; Lagrou, Katrien; Meersseman, Philippe; Neyts, Johan; Thal, Dietmar Rudolf; Topsakal, Vedat; Vandenbriele, Christophe; Wauters, Joost; Mombaerts, Peter; Van Gerven, Laura.
  • Khan M; Max Planck Research Unit for Neurogenetics, Frankfurt, Germany.
  • Clijsters M; Department of Neurosciences, Experimental Otorhinolaryngology, Rhinology Research, KU Leuven, Leuven, Belgium.
  • Choi S; Max Planck Research Unit for Neurogenetics, Frankfurt, Germany.
  • Backaert W; Department of Otorhinolaryngology, Head and Neck Surgery, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, KU Leuven, Leuven, Belgium.
  • Claerhout M; Department of Otorhinolaryngology, Head and Neck Surgery, University Hospitals Leuven, Leuven, Belgium.
  • Couvreur F; Department of Otorhinolaryngology, Head and Neck Surgery, University Hospitals Leuven, Leuven, Belgium.
  • Van Breda L; Department of Otorhinolaryngology, Head and Neck Surgery, University Hospitals Leuven, Leuven, Belgium.
  • Bourgeois F; Department of Otorhinolaryngology, Head and Neck Surgery, AZ Sint-Jan Brugge-Oostende AV, Bruges, Belgium.
  • Speleman K; Department of Otorhinolaryngology, Head and Neck Surgery, AZ Sint-Jan Brugge-Oostende AV, Bruges, Belgium.
  • Klein S; Department of Neurosurgery, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium.
  • Van Laethem J; Department of Infectious Diseases, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium.
  • Verstappen G; Department of Otorhinolaryngology - Head and Neck Surgery, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium.
  • Dereli AS; Max Planck Research Unit for Neurogenetics, Frankfurt, Germany.
  • Yoo SJ; Max Planck Research Unit for Neurogenetics, Frankfurt, Germany; Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, Republic of Korea.
  • Zhou H; Max Planck Research Unit for Neurogenetics, Frankfurt, Germany.
  • Dan Do TN; Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, KU Leuven, Leuven, Belgium.
  • Jochmans D; Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, KU Leuven, Leuven, Belgium.
  • Laenen L; Department of Laboratory Medicine & National Reference Center for Respiratory Pathogens, University Hospitals Leuven, Leuven, Belgium.
  • Debaveye Y; Intensive Care Medicine, University Hospitals Leuven, Leuven, Belgium; Department of Cellular and Molecular Medicine, Laboratory of Intensive Care Medicine, KU Leuven, Leuven, Belgium.
  • De Munter P; Department of General Internal Medicine, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Laboratory for Clinical Infectious and Inflammatory Disorders, KU Leuven, Leuven, Belgium.
  • Gunst J; Intensive Care Medicine, University Hospitals Leuven, Leuven, Belgium; Department of Cellular and Molecular Medicine, Laboratory of Intensive Care Medicine, KU Leuven, Leuven, Belgium.
  • Jorissen M; Department of Neurosciences, Experimental Otorhinolaryngology, Rhinology Research, KU Leuven, Leuven, Belgium; Department of Otorhinolaryngology, Head and Neck Surgery, University Hospitals Leuven, Leuven, Belgium.
  • Lagrou K; Department of Laboratory Medicine & National Reference Center for Respiratory Pathogens, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical Bacteriology and Mycology, KU Leuven, Leuven, Belgium.
  • Meersseman P; Department of General Internal Medicine, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Laboratory for Clinical Infectious and Inflammatory Disorders, KU Leuven, Leuven, Belgium.
  • Neyts J; Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, KU Leuven, Leuven, Belgium.
  • Thal DR; Department of Pathology, University Hospitals Leuven, Leuven, Belgium; Laboratory of Neuropathology, Department of Imaging & Pathology and Leuven Brain Institute, KU Leuven, Leuven, Belgium.
  • Topsakal V; Department of Otorhinolaryngology - Head and Neck Surgery, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium.
  • Vandenbriele C; Department of Cardiovascular Diseases, University Hospitals Leuven, Leuven, Belgium; Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium.
  • Wauters J; Department of General Internal Medicine, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Laboratory for Clinical Infectious and Inflammatory Disorders, KU Leuven, Leuven, Belgium.
  • Mombaerts P; Max Planck Research Unit for Neurogenetics, Frankfurt, Germany. Electronic address: peter.mombaerts@gen.mpg.de.
  • Van Gerven L; Department of Neurosciences, Experimental Otorhinolaryngology, Rhinology Research, KU Leuven, Leuven, Belgium; Department of Otorhinolaryngology, Head and Neck Surgery, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immu
Neuron ; 2022 Nov 10.
Article in English | MEDLINE | ID: covidwho-2105658
ABSTRACT
Can SARS-CoV-2 hitchhike on the olfactory projection and take a direct and short route from the nose into the brain? We reasoned that the neurotropic or neuroinvasive capacity of the virus, if it exists, should be most easily detectable in individuals who died in an acute phase of the infection. Here, we applied a postmortem bedside surgical procedure for the rapid procurement of tissue, blood, and cerebrospinal fluid samples from deceased COVID-19 patients infected with the Delta, Omicron BA.1, or Omicron BA.2 variants. Confocal imaging of sections stained with fluorescence RNAscope and immunohistochemistry afforded the light-microscopic visualization of extracellular SARS-CoV-2 virions in tissues. We failed to find evidence for viral invasion of the parenchyma of the olfactory bulb and the frontal lobe of the brain. Instead, we identified anatomical barriers at vulnerable interfaces, exemplified by perineurial olfactory nerve fibroblasts enwrapping olfactory axon fascicles in the lamina propria of the olfactory mucosa.
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Full text: Available Collection: International databases Database: MEDLINE Topics: Variants Language: English Journal subject: Neurology Year: 2022 Document Type: Article Affiliation country: J.neuron.2022.11.007

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Full text: Available Collection: International databases Database: MEDLINE Topics: Variants Language: English Journal subject: Neurology Year: 2022 Document Type: Article Affiliation country: J.neuron.2022.11.007