Photoactive Conjugated Microporous Polymer/Carbon Nanotube Coupled with T-junction Recycling Dual-Strand Displacement Amplification for Sensing N-Gene of COVID-19
Sensors and Actuators B: Chemical
; : 132974, 2022.
Article
in English
| ScienceDirect | ID: covidwho-2105983
ABSTRACT
In this work, a novel conjugated microporous polymer on carbon nanotube composite (CMP-CNTs) was synthesized as photoelectrochemical (PEC) signal probe to construct sensitive PEC biosensor for sensing N-Gene of COVID-19 by integrating with an ingenious target-trigger T-junction recycling dual-strand displacement amplification (T-DSDA). The CMP-CNTs composites has an ideal photoelectrical conversion efficiency owing to the appearance of a good band matching that can effectively promote electron-hole pairs separation and accelerate carrier migration, thereby generating an extremely high initial photocurrent. Meanwhile, the T-DSDA with superior target conversion efficiency to traditional approaches could convert the small number of targets into extensive output DNAs, leading to the in-situ generation of quench agent N-GQDS decorated nanowires on electrode for significantly reducing initial photocurrent. The results demonstrated that proposed PEC biosensor had a high sensitivity towards N-Gene of COVID-19 and the detection limit was 33 aM, which provided a new way to build the simple, sensitive, and reliable sensing platform for great potential in biological analysis and early clinical diagnosis.
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Databases of international organizations
Database:
ScienceDirect
Language:
English
Journal:
Sensors and Actuators B: Chemical
Year:
2022
Document Type:
Article
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