Protocol for high-throughput screening of SARS-CoV-2 main protease inhibitors using a robust fluorescence polarization assay.
STAR Protoc
; 3(4): 101794, 2022 12 16.
Article
in English
| MEDLINE | ID: covidwho-2106167
ABSTRACT
Discovery of efficacious antiviral agents targeting SARS-CoV-2 main protease (Mpro) is of the highest importance to fight against COVID-19. Here, we describe a simple protocol for high-throughput screening of Mpro inhibitors using a robust fluorescence polarization (FP) assay. Candidate Mpro inhibitors from large compound libraries could be rapidly identified by monitoring the change of millipolarization unit value. This affordable FP assay can be modified to screen antiviral agents targeting virus protease. For complete details on the use and execution of this protocol, please refer to Li et al. (2022), Yan et al. (2021), and Yan et al. (2022c).
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
High-Throughput Screening Assays
/
COVID-19 Drug Treatment
Limits:
Humans
Language:
English
Journal:
STAR Protoc
Year:
2022
Document Type:
Article
Affiliation country:
J.xpro.2022.101794
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