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Protocol for high-throughput screening of SARS-CoV-2 main protease inhibitors using a robust fluorescence polarization assay.
Zhang, Jing; Yan, Haohao; Yan, Gangan; Liu, Xiaoping; Wang, Yanchang; Chen, Yunyu.
  • Zhang J; Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
  • Yan H; Institute for Drug Screening and Evaluation, Wannan Medical College, Wuhu 241002, China.
  • Yan G; Institute for Drug Screening and Evaluation, Wannan Medical College, Wuhu 241002, China.
  • Liu X; Institute for Drug Screening and Evaluation, Wannan Medical College, Wuhu 241002, China.
  • Wang Y; Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL 32306, USA.
  • Chen Y; Institute for Drug Screening and Evaluation, Wannan Medical College, Wuhu 241002, China.
STAR Protoc ; 3(4): 101794, 2022 12 16.
Article in English | MEDLINE | ID: covidwho-2106167
ABSTRACT
Discovery of efficacious antiviral agents targeting SARS-CoV-2 main protease (Mpro) is of the highest importance to fight against COVID-19. Here, we describe a simple protocol for high-throughput screening of Mpro inhibitors using a robust fluorescence polarization (FP) assay. Candidate Mpro inhibitors from large compound libraries could be rapidly identified by monitoring the change of millipolarization unit value. This affordable FP assay can be modified to screen antiviral agents targeting virus protease. For complete details on the use and execution of this protocol, please refer to Li et al. (2022), Yan et al. (2021), and Yan et al. (2022c).
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Full text: Available Collection: International databases Database: MEDLINE Main subject: High-Throughput Screening Assays / COVID-19 Drug Treatment Limits: Humans Language: English Journal: STAR Protoc Year: 2022 Document Type: Article Affiliation country: J.xpro.2022.101794

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Full text: Available Collection: International databases Database: MEDLINE Main subject: High-Throughput Screening Assays / COVID-19 Drug Treatment Limits: Humans Language: English Journal: STAR Protoc Year: 2022 Document Type: Article Affiliation country: J.xpro.2022.101794